Abstract

Methodology for the determination of the structure of the polar, poly functional aromatic compounds produced upon oxidative pyrolysis or incomplete combustion will be developed. Because of the expected complexity of the mixtures to be studied, high pressure liquid chromatography must be used for at least partial separation. For the determination of the structure of the components, high resolution tandem mass spectrometry will be employed. For this purpose the deposition device previously designed for the recording of infrared spectra of components eluting from an HPLC will be adapted to the mass spectrometer. Successful implementation of this design will permit the determination of the infrared spectra, conventional high resolution mass spectra, and the mass spectra produced upon collision induced decomposition of the same fraction of HPLC eluent.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Research Program Projects (P01)
Project #
5P01ES001640-14
Application #
3855565
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
14
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
Massachusetts Institute of Technology
Department
Type
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02139

  Publications

Tomita-Mitchell, Aoy; Ling, Losee Lucy; Glover, Curtis L et al. (2003) The mutational spectrum of the HPRT gene from human T cells in vivo shares a significant concordant set of hot spots with MNNG-treated human cells. Cancer Res 63:5793-8
Tomita-Mitchell, A; Kat, A G; Marcelino, L A et al. (2000) Mismatch repair deficient human cells: spontaneous and MNNG-induced mutational spectra in the HPRT gene. Mutat Res 450:125-38
Durant, J L; Lafleur, A L; Busby Jr, W F et al. (1999) Mutagenicity of C24H14 PAH in human cells expressing CYP1A1. Mutat Res 446:1-14
Wang, J S; He, X; Mulder, P P et al. (1999) Comparative tumorigenicity of the cyclopenta-fused polycyclic aromatic hydrocarbons aceanthrylene, dihydroaceanthrylene and acephenanthrylene in preweanling CD-1 and BLU:Ha mouse bioassays. Carcinogenesis 20:1137-41
Busby Jr, W F; Smith, H; Crespi, C L et al. (1997) Mutagenicity of the atmospheric transformation products 2-nitrofluoranthene and 2-nitrodibenzopyranone in Salmonella and human cell forward mutation assays. Mutat Res 389:261-70
Busby Jr, W F; Smith, H; Plummer, E F et al. (1997) Mutagenicity of cyclopenta-fused polynuclear aromatic hydrocarbons and a non-polar fraction from a fuel combustion sample in a Salmonella forward mutation assay without exogenous metabolic activation. Mutat Res 391:117-25
Durant, J L; Busby Jr, W F; Lafleur, A L et al. (1996) Human cell mutagenicity of oxygenated, nitrated and unsubstituted polycyclic aromatic hydrocarbons associated with urban aerosols. Mutat Res 371:123-57
Palotas, A B; Rainey, L C; Feldermann, C J et al. (1996) Soot morphology: an application of image analysis in high-resolution transmission electron microscopy. Microsc Res Tech 33:266-78
Wang, J S; Busby Jr, W F (1996) Bacterial and human cell mutagenicity and mouse lung tumorigenicity of the oxygenated polynuclear aromatic hydrocarbon phenalenone. Fundam Appl Toxicol 33:212-9
Busby Jr, W F; Smith, H; Crespi, C L et al. (1995) Mutagenicity of benzo[a]pyrene and dibenzopyrenes in the Salmonella typhimurium TM677 and the MCL-5 human cell forward mutation assays. Mutat Res 342:9-16

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