The purpose of the program is study of the physical and chemical conditions in combustion systems that lead to the formation of fine organic and inorganic particles and the coating of these particles with organic mutagens that may be present in combustion gases, and to assess the effects of the particles, with and without mutagens, on mutation of a human lymphoblast cell strain. Particles will be generated in furnaces in which soot and/or selected metals, such as cadmium, zinc, manganese and vanadium, are volatilized, oxidized, and possibly sulfated for conditions simulating those encountered during combustion of fuels. On cooling of the combustion gases, selected mutagenitic compounds that can be formed in combustion systems will be condensed on the particles. In a second set of experiments, organic and inorganic aerosols will be formed by combustion of coals and synthetic carbon spheres doped with selected metal-bearing compounds. These particles will also be coated with mutagenic compounds. The physical and chemical characteristics of particles form the two sources to be determined by a number of critical methods. Then particles generated in the equipment will be collected, and the effect of mutagen-particle interaction on mutation of a human lymphoblast strain will be assessed.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Research Program Projects (P01)
Project #
5P01ES001640-14
Application #
3855569
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
14
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Massachusetts Institute of Technology
Department
Type
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02139
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Durant, J L; Busby Jr, W F; Lafleur, A L et al. (1996) Human cell mutagenicity of oxygenated, nitrated and unsubstituted polycyclic aromatic hydrocarbons associated with urban aerosols. Mutat Res 371:123-57
Palotas, A B; Rainey, L C; Feldermann, C J et al. (1996) Soot morphology: an application of image analysis in high-resolution transmission electron microscopy. Microsc Res Tech 33:266-78
Wang, J S; Busby Jr, W F (1996) Bacterial and human cell mutagenicity and mouse lung tumorigenicity of the oxygenated polynuclear aromatic hydrocarbon phenalenone. Fundam Appl Toxicol 33:212-9
Wang, J S; Busby, W F; Wogan, G N (1995) Tissue distribution of DNA adducts in pre-weanling BLU:Ha mice treated with a tumorigenic dose of fluoranthene. Cancer Lett 92:9-19

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