a pure sample as a standard. New peptide adducts formed by AL-II have been isolated by HPLC using a fluorescence detector and analyzed by MALDI mass spectrometry. A brief report of each of these advances is found in the Progress Report/Preliminary Studies section. Finally, Dr. Iden, seeking to improve access to state-of-the-art mass spectrometry techniques, submitted a proposal for a new triple quadrupole mass spectrometer system to the NIH National Center for Research Resources. Council is scheduled to meet in October, 2006. If this instrument is acquired in the Spring, 2007, as scheduled, levels of sensitivity for quantitative detection of DNA and protein adducts will be increased by a factor of ten over that attained by our current triple quadrupole instrumentation. A.
Specific Aims Oligodeoxynucleotide synthesis and chemical analysis performed by the Core Facility provides fundamental support for all Program research. The Core will provide modified and normal oligomers to all investigators. DNA synthesis personnel will work closely with synthetic chemists to test new, modified deoxynucleosides phosphoramidites and incorporate them into DNA oligomers. We will refine our quantitative mass spectrometer methods and proteomics capabilities and focus on the development of sensitive, new techniques to identify DNA and protein adducts formed by the aristolochic acids (AAs) and their metabolites.
The specific aims of the Core Facility are: 1. Toprepare
|Yun, Byeong Hwa; Guo, Jingshu; Turesky, Robert J (2018) Formalin-Fixed Paraffin-Embedded Tissues-An Untapped Biospecimen for Biomonitoring DNA Adducts by Mass Spectrometry. Toxics 6:|
|Jelakovi?, Bojan; Vukovi? Lela, Ivana; Karanovi?, Sandra et al. (2015) Chronic dietary exposure to aristolochic acid and kidney function in native farmers from a Croatian endemic area and Bosnian immigrants. Clin J Am Soc Nephrol 10:215-23|
|Romanov, Victor; Whyard, Terry C; Waltzer, Wayne C et al. (2015) Aristolochic acid-induced apoptosis and G2 cell cycle arrest depends on ROS generation and MAP kinases activation. Arch Toxicol 89:47-56|
|Yun, Byeong Hwa; Sidorenko, Viktoriya S; Rosenquist, Thomas A et al. (2015) New Approaches for Biomonitoring Exposure to the Human Carcinogen Aristolochic Acid. Toxicol Res (Camb) 4:763-776|
|Castells, Xavier; Karanovi?, Sandra; Ardin, Maude et al. (2015) Low-Coverage Exome Sequencing Screen in Formalin-Fixed Paraffin-Embedded Tumors Reveals Evidence of Exposure to Carcinogenic Aristolochic Acid. Cancer Epidemiol Biomarkers Prev 24:1873-81|
|Jelakovi?, Bojan; Nikoli?, Jovan; Radovanovi?, Zoran et al. (2014) Consensus statement on screening, diagnosis, classification and treatment of endemic (Balkan) nephropathy. Nephrol Dial Transplant 29:2020-7|
|Ivkovi?, Vanja; Karanovi?, Sandra; Fištrek Prli?, Margareta et al. (2014) Is herbal tea consumption a factor in endemic nephropathy? Eur J Epidemiol 29:221-4|
|Yun, Byeong Hwa; Yao, Lihua; Jelakovi?, Bojan et al. (2014) Formalin-fixed paraffin-embedded tissue as a source for quantitation of carcinogen DNA adducts: aristolochic acid as a prototype carcinogen. Carcinogenesis 35:2055-61|
|Attaluri, Sivaprasad; Iden, Charles R; Bonala, Radha R et al. (2014) Total synthesis of the aristolochic acids, their major metabolites, and related compounds. Chem Res Toxicol 27:1236-42|
|Sidorenko, Viktoriya S; Attaluri, Sivaprasad; Zaitseva, Irina et al. (2014) Bioactivation of the human carcinogen aristolochic acid. Carcinogenesis 35:1814-22|
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