This laboratory will prepare and purify deoxyoligonucleotides for the various investigators. The facility will have two functions: preparation of unmodified oligomers derived from the four natural deoxynucleosides and preparation of carcinogen-modified oligomers or halogen-substituted oligomers for use in substitution reactions. Small quantities of the unmodified oligomers will be needed for genetics experiments; the facility will also prepare the larger quantities needed for NMR experiments. While the preparation of normal oligomers might be contracted out to commercial laboratories, the irreplaceable function of the laboratory will be to prepare structurally modified oligomers using novel reagents developed in Project 1. In some case only minute amounts of adducted oligomers will be required, i.e., for molecular biological experiments, but much of the proposed work will require relatively large quantities. The NMR experiments require milligram quantities since useful NOESY spectra generally require at least 2-4 mM concentrations in a volume of 0.4 mL; similarly X-ray diffraction studies require relatively large quantities for preparation of crystals.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Research Program Projects (P01)
Project #
2P01ES005355-08
Application #
6271150
Study Section
Project Start
1998-08-01
Project End
1999-07-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
8
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Vanderbilt University Medical Center
Department
Type
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212
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