The analytical core facilities have been developed to provide a strong central analytical core, with a variety of instrumentation for quantitative analysis, for use for all investigators in the program project. The core provides facilities and training that is used by each of the projects as required, for computerized image analysis of autoradiographic data such as gels, radioligand binding and in the situ hybridization, quantitative morphometric analysis of immunolabeled and conventionally stained tissue sections, and fluorescence ratio imaging. Three computerized image analysis systems are available for densitometric analysis for macro-autoradiographic specimens, a Zeiss Kontron KS400 Image Analysis System, which is used primarily for quantitative morphometric analysis of brightfield and fluorescence microscope images, and a Zeiss Attofluar Calcium Imaging System. A Fuji BAS 2500 Phosphor Imaging Scanner will be added to the MCID/M4 system to enhance the quantitative capabilities of the core; this scanner will be utilized by all of the proposed projects. Two darkrooms are also provided in the core A. Dr. Wilson, core leader, will assist investigator with the design and implementation of computer image analysis protocols and set policies for shared use of the facilities. Analysis of lead levels in the blood and tissue samples is provided through core A for all investigators, in the trace metals laboratory. Three methods of analysis are available; atomic absorption spectroscopy will be the primary method used by the program projects. Dr. Bressler, Associate Director of the trace metals laboratory, will provide oversight of protocols for lead administration and blood/tissue lead analysis. Core A will thus provide an important service, determination of lead levels, and provide access to state-of-the-art image analysis equipment that will be used for a wide variety of quantitative assessments by individual projects.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Research Program Projects (P01)
Project #
5P01ES008131-03
Application #
6301550
Study Section
Project Start
2000-04-01
Project End
2001-03-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
3
Fiscal Year
2000
Total Cost
$155,404
Indirect Cost
Name
Hugo W. Moser Research Institute Kennedy Krieger
Department
Type
DUNS #
167202410
City
Baltimore
State
MD
Country
United States
Zip Code
21205
Cheong, Jae Hoon; Bannon, Desmond; Olivi, Luisa et al. (2004) Different mechanisms mediate uptake of lead in a rat astroglial cell line. Toxicol Sci 77:334-40
Hossain, Mir Ahamed; Russell, Juliet C; Miknyoczki, Sheila et al. (2004) Vascular endothelial growth factor mediates vasogenic edema in acute lead encephalopathy. Ann Neurol 55:660-7
Patra, Ramesh C; Blue, Mary E; Johnston, Michael V et al. (2004) Activity-dependent expression of Egr1 mRNA in somatosensory cortex of developing rats. J Neurosci Res 78:235-44
Johnston, Michael V (2004) Clinical disorders of brain plasticity. Brain Dev 26:73-80
Bannon, Desmond I; Abounader, Roger; Lees, Peter S J et al. (2003) Effect of DMT1 knockdown on iron, cadmium, and lead uptake in Caco-2 cells. Am J Physiol Cell Physiol 284:C44-50
Glenn, Barbara S; Stewart, Walter F; Links, Jonathan M et al. (2003) The longitudinal association of lead with blood pressure. Epidemiology 14:30-6
Olivi, Luisa; Sisk, Jeanne; Bressler, Joseph (2003) The involvement of lipid activators of protein kinase C in the induction of ZIF268 in PC12 cells exposed to lead. Neurochem Res 28:65-71
Johnston, Michael V (2003) Injury and plasticity in the developing brain. Exp Neurol 184 Suppl 1:S37-41
Donovan, Stacy L; Mamounas, Laura A; Andrews, Anne M et al. (2002) GAP-43 is critical for normal development of the serotonergic innervation in forebrain. J Neurosci 22:3543-52
Kim, Kyung-Ah; Chakraborti, Tamal; Goldstein, Gary et al. (2002) Exposure to lead elevates induction of zif268 and Arc mRNA in rats after electroconvulsive shock: the involvement of protein kinase C. J Neurosci Res 69:268-77

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