Abstract

The aims of this core are to develop PCR-based assays to measure selected transcript concentration change in animals or tissues treated with botanicals, extract and store DNA from botanicals, identify variation in nucleotide sequences of botanicals, quantify DNA in botanical samples, and use """"""""TaqMan"""""""" technology to devise method to identify impurities in the DNA extracts. Most of these aims will be accomplished using the new ABI TaqMan technology and an ABI Prism 7700 Sequence Detector and traditional sequence analysis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Research Program Projects (P01)
Project #
5P01ES010535-03
Application #
6575708
Study Section
Special Emphasis Panel (ZRG1)
Project Start
2002-03-01
Project End
2003-02-28
Budget Start
Budget End
Support Year
3
Fiscal Year
2002
Total Cost
$65,394
Indirect Cost

  Institution

Name
University of Missouri-Columbia
Department
Type
DUNS #
112205955
City
Columbia
State
MO
Country
United States
Zip Code
65211

  Publications

Lei, Wei; Browning Jr, Jimmy D; Eichen, Peggy A et al. (2015) Immuno-stimulatory activity of a polysaccharide-enriched fraction of Sutherlandia frutescens occurs by the toll-like receptor-4 signaling pathway. J Ethnopharmacol 172:247-53
Slusarz, Anna; Jackson, Glenn A; Day, J Kevin et al. (2012) Aggressive prostate cancer is prevented in ER?KO mice and stimulated in ER?KO TRAMP mice. Endocrinology 153:4160-70
Brown, Marybeth; Ning, Jie; Ferreira, J Andries et al. (2009) Estrogen receptor-alpha and -beta and aromatase knockout effects on lower limb muscle mass and contractile function in female mice. Am J Physiol Endocrinol Metab 296:E854-61
Richter, Catherine A; Taylor, Julia A; Ruhlen, Rachel L et al. (2007) Estradiol and Bisphenol A stimulate androgen receptor and estrogen receptor gene expression in fetal mouse prostate mesenchyme cells. Environ Health Perspect 115:902-8
Zhou, Wei; Lo, Shih-Ching; Liu, Jing-Hua et al. (2007) ERRbeta: a potent inhibitor of Nrf2 transcriptional activity. Mol Cell Endocrinol 278:52-62
Curran, Edward M; Tassell, Audrey Hart-Van; Judy, Barbara M et al. (2007) Estrogen increases menopausal host susceptibility to experimental ascending urinary-tract infection. J Infect Dis 195:680-3
Wang, Qun; Tompkins, Kenneth D; Simonyi, Agnes et al. (2006) Apocynin protects against global cerebral ischemia-reperfusion-induced oxidative stress and injury in the gerbil hippocampus. Brain Res 1090:182-9
Zhou, Wei; Liu, Zhilin; Wu, Jianbo et al. (2006) Identification and characterization of two novel splicing isoforms of human estrogen-related receptor beta. J Clin Endocrinol Metab 91:569-79
Wang, Qun; Yu, Sue; Simonyi, Agnes et al. (2005) Kainic acid-mediated excitotoxicity as a model for neurodegeneration. Mol Neurobiol 31:3-16
Lambert, K Chad; Curran, Edward M; Judy, Barbara M et al. (2005) Estrogen receptor alpha (ERalpha) deficiency in macrophages results in increased stimulation of CD4+ T cells while 17beta-estradiol acts through ERalpha to increase IL-4 and GATA-3 expression in CD4+ T cells independent of antigen presentation. J Immunol 175:5716-23

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