The Molecular Imaging Resource core will support the overall Program Project by housing resources and expertise necessary to prepare and introduce labeled proteins and proteins analogues into living cells, and then analyzing the spatial and kinetic distribution of these reagents and endogenous proteins as well as effects of these reagents on cell morphology, architecture and behavior. All projects of this program will make use of the activities of this core. The functions of this core include protein purification and derivitization with fluorescent tracers, introduction of proteins and expression vectors by microinjection, fluorescence recovery after photobleaching analysis, low light level time-lapse fluorescence imaging of labeled proteins and GFP-fusion proteins, immunolocalization of endogenous proteins and introduced reagents, computer-assisted analysis of cell shape and motility behavior, and 3-dimensional analysis and reconstruction of cell morphology and cytoskeletal architecture. Resources are available allowing these techniques to be applied to large cell populations or individual living cells.
The specific aims of the Molecular Cytology Core are to: 1) develop and produce derivitized analogues of actin, 2) provide equipment, expertise and training for introducing macromolecules and expression vectors into living cells, and 3) provide technical assistance and training in computer assisted imaging techniques used to analyze static and dynamic distributions of cytoskeletal proteins in 2- and 3-dimensions.

National Institute of Health (NIH)
National Institute of Environmental Health Sciences (NIEHS)
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University of Michigan Ann Arbor
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Sun, Xiankui; Fontaine, Jean-Marc; Hoppe, Adam D et al. (2010) Abnormal interaction of motor neuropathy-associated mutant HspB8 (Hsp22) forms with the RNA helicase Ddx20 (gemin3). Cell Stress Chaperones 15:567-82
Sun, Xiankui; Fontaine, Jean-Marc; Bartl, Ingrid et al. (2007) Induction of Hsp22 (HspB8) by estrogen and the metalloestrogen cadmium in estrogen receptor-positive breast cancer cells. Cell Stress Chaperones 12:307-19
Simon, Stephanie; Fontaine, Jean-Marc; Martin, Jody L et al. (2007) Myopathy-associated alphaB-crystallin mutants: abnormal phosphorylation, intracellular location, and interactions with other small heat shock proteins. J Biol Chem 282:34276-87
Eichler, Tad; Ma, Qing; Kelly, Caitlin et al. (2006) Single and combination toxic metal exposures induce apoptosis in cultured murine podocytes exclusively via the extrinsic caspase 8 pathway. Toxicol Sci 90:392-9
Sun, Xiankui; Welsh, Michael J; Benndorf, Rainer (2006) Conformational changes resulting from pseudophosphorylation of mammalian small heat shock proteins--a two-hybrid study. Cell Stress Chaperones 11:61-70
Fontaine, Jean-Marc; Sun, Xiankui; Hoppe, Adam D et al. (2006) Abnormal small heat shock protein interactions involving neuropathy-associated HSP22 (HSPB8) mutants. FASEB J 20:2168-70
Eichler, Tad E; Ransom, Richard F; Smoyer, William E (2005) Differential induction of podocyte heat shock proteins by prolonged single and combination toxic metal exposure. Toxicol Sci 84:120-8
Yancy, Shannon L; Shelden, Eric A; Gilmont, Robert R et al. (2005) Sodium arsenite exposure alters cell migration, focal adhesion localization and decreases tyrosine phosphorylation of focal adhesion kinase in H9C2 myoblasts. Toxicol Sci 84:278-86
Fontaine, Jean-Marc; Sun, Xiankui; Benndorf, Rainer et al. (2005) Interactions of HSP22 (HSPB8) with HSP20, alphaB-crystallin, and HSPB3. Biochem Biophys Res Commun 337:1006-11
Hirano, Sahoko; Sun, Xiankui; DeGuzman, Cheryl A et al. (2005) p38 MAPK/HSP25 signaling mediates cadmium-induced contraction of mesangial cells and renal glomeruli. Am J Physiol Renal Physiol 288:F1133-43

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