The mission of the High-Throughput Screening and Chemical Library (HTSCL) Core is to support the high-throughput screening, medicinal chemistry, automated microscopy and image analysis needs of the individual Projects. The Burnham Institute for Medical Research (BIMR) has collaborated with neighboring academic institutions to establish the San Diego Center for Chemical Genomics (SDCCG). The SDCCG will provide the projects access to state-of-the-art robotics and instrumentation for HTS for the purpose of discovering novel therapeutics for Parkinson's disease (PD), which will be a major function of Core E. HTS will be conducted in vitro against biochemical targets or cellular responses (Projects 2 3, and 4). A second major function of the core will be the automated acquisition and analysis of images of cells to quantify marker or phenotypic responses (Projects 2 and 3) using the sophisticated and proprietary automated microscopy platforms and algorithms developed within the center. In addition, the core will assume the responsibility of converting primary validated hits to leads through medicinal chemistry and nuclear magnetic resonance (NMR)-based and computational modeling strategies for compound optimization (Projects 2, 3, and 4). Core E will provide these functions for the Parkinson's Disease (PD) researchers in this Center.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Research Program Projects (P01)
Project #
5P01ES016738-04
Application #
8292295
Study Section
Special Emphasis Panel (ZES1)
Project Start
2011-07-01
Project End
2013-06-30
Budget Start
2011-07-01
Budget End
2012-06-30
Support Year
4
Fiscal Year
2011
Total Cost
$123,803
Indirect Cost
Name
Sanford-Burnham Medical Research Institute
Department
Type
DUNS #
020520466
City
La Jolla
State
CA
Country
United States
Zip Code
92037
Oh, Chang-Ki; Sultan, Abdullah; Platzer, Joseph et al. (2017) S-Nitrosylation of PINK1 Attenuates PINK1/Parkin-Dependent Mitophagy in hiPSC-Based Parkinson's Disease Models. Cell Rep 21:2171-2182
Singec, Ilyas; Crain, Andrew M; Hou, Junjie et al. (2016) Quantitative Analysis of Human Pluripotency and Neural Specification by In-Depth (Phospho)Proteomic Profiling. Stem Cell Reports 7:527-542
Qu, Zhe; Greenlief, C Michael; Gu, Zezong (2016) Quantitative Proteomic Approaches for Analysis of Protein S-Nitrosylation. J Proteome Res 15:1-14
Satoh, Takumi; Stalder, Romain; McKercher, Scott R et al. (2015) Nrf2 and HSF-1 Pathway Activation via Hydroquinone-Based Proelectrophilic Small Molecules is Regulated by Electrochemical Oxidation Potential. ASN Neuro 7:
Spiering, Sean; Davidovics, Herman; Bushway, Paul J et al. (2015) High content screening for modulators of cardiac differentiation in human pluripotent stem cells. Methods Mol Biol 1263:43-61
Okamoto, Shu-ichi; Lipton, Stuart A (2015) S-Nitrosylation in neurogenesis and neuronal development. Biochim Biophys Acta 1850:1588-93
Zhou, Hui; Qu, Zhe; Mossine, Valeri V et al. (2014) Proteomic analysis of the effects of aged garlic extract and its FruArg component on lipopolysaccharide-induced neuroinflammatory response in microglial cells. PLoS One 9:e113531
Okamoto, Shu-Ichi; Nakamura, Tomohiro; Cieplak, Piotr et al. (2014) S-nitrosylation-mediated redox transcriptional switch modulates neurogenesis and neuronal cell death. Cell Rep 8:217-28
Chan, Shing Fai; Sances, Sam; Brill, Laurence M et al. (2014) ATM-dependent phosphorylation of MEF2D promotes neuronal survival after DNA damage. J Neurosci 34:4640-53
Qu, Zhe; Meng, Fanjun; Zhou, Hui et al. (2014) NitroDIGE analysis reveals inhibition of protein S-nitrosylation by epigallocatechin gallates in lipopolysaccharide-stimulated microglial cells. J Neuroinflammation 11:17

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