Abstract

The program is an interdisciplinary study of several membrane proteins utilizing in common an electron microscope facility and a laboratory for microsequencing in which the capability for nanomole and subnanomole level analyses are being developed. Research will focus on the structure and physiology of gap junctions and their constituent proteins, the nature of protein diversity in transplantation antigens and in the T/t complex so as to provide insight into the organization and evolution of the genes that code for the surface molecules. Work on the glycoproteins of the membranes of the Sindbis virus and their precursors and immunocytological and biochemical analyses of filamentous proteins in muscle and non-muscle cells is being pursued as well as analyses characterizing membrane proteins from cloned murine cell lines derived from UV-induced skin tumors bearing both specific and cross-reactive antigens.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
5P01GM006965-26
Application #
3096001
Study Section
(SSS)
Project Start
1979-03-01
Project End
1986-02-28
Budget Start
1985-03-01
Budget End
1986-02-28
Support Year
26
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
California Institute of Technology
Department
Type
Schools of Arts and Sciences
DUNS #
078731668
City
Pasadena
State
CA
Country
United States
Zip Code
91125

  Publications

Manjunath, C K; Nicholson, B J; Teplow, D et al. (1987) The cardiac gap junction protein (Mr 47,000) has a tissue-specific cytoplasmic domain of Mr 17,000 at its carboxy-terminus. Biochem Biophys Res Commun 142:228-34
Dermietzel, R; Yancey, B; Janssen-Timmen, U et al. (1987) Simultaneous light and electron microscopic observation of immunolabeled liver 27 KD gap junction protein on ultra-thin cryosections. J Histochem Cytochem 35:387-92
Revel, J P; Yancey, S B; Nicholson, B et al. (1987) Sequence diversity of gap junction proteins. Ciba Found Symp 125:108-27
Dermietzel, R; Yancey, S B; Traub, O et al. (1987) Major loss of the 28-kD protein of gap junction in proliferating hepatocytes. J Cell Biol 105:1925-34
Normanly, J; Ogden, R C; Horvath, S J et al. (1986) Changing the identity of a transfer RNA. Nature 321:213-9
Lopez, S; Bell, J R; Strauss, E G et al. (1985) The nonstructural proteins of Sindbis virus as studied with an antibody specific for the C terminus of the nonstructural readthrough polyprotein. Virology 141:235-47
Mayne, J T; Bell, J R; Strauss, E G et al. (1985) Pattern of glycosylation of Sindbis virus envelope proteins synthesized in hamster and chicken cells. Virology 142:121-33
Springer, T A; Teplow, D B; Dreyer, W J (1985) Sequence homology of the LFA-1 and Mac-1 leukocyte adhesion glycoproteins and unexpected relation to leukocyte interferon. Nature 314:540-2
Nicholson, B J; Gros, D B; Kent, S B et al. (1985) The Mr 28,000 gap junction proteins from rat heart and liver are different but related. J Biol Chem 260:6514-7
Revel, J P; Nicholson, B J; Yancey, S B (1985) Chemistry of gap junctions. Annu Rev Physiol 47:263-79

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