We are involved in studying the structure and regulation of eukaryotic genes, using Drosophila as an experimental object. Although we come from diverse background (genetics, molecular biology, developmental biology, population genetics, cell biology), we intersect in our common interest: to use the genetically most favorable higher organism, Drosophila, and the current techniques of molecular and cellular biology, to illuminate how the genes of higher are organized, evolve, and are regulated during development and during the cell cycle. Indeed, a strength of our group is that it represents complementary and yet overlapping approaches. In the context of the program project, we intend to: (a) Test by genetics the in vivo function of transcription factors putatively involved in the developmental regulation of chorion genes, and isolate additional components involved in chorion regulation using enhancer trap method. (b) Study and manipulate the hobo mobile elements, including characterization of the transposase transcript and the mechanism of transposition of the element in the germ line; develop novel transformation vector and procedures. (c) Characterize DNA polymorphism and evolution of the decapentaplegic gene in populations of D. melanogaster and D. pseudoobscura, to obtain the first data on selective constraints on amino acid sequences, message structure and cis-regulatory elements for a gene controlling basic morphogenetic processes. (d) Use genetic approaches to understanding the control of embryonic muscle cell commitment and differentiation, focusing on the nau gene that encodes basic and helix-loop-lelix domains similar to those of vertebrate myogenic regulatory factors. (e) Study the in vitro properties and in vivo function of the products of two genes, ned and nod, which belong to the kinesin superfamily and movements of microtubules in the meiotic and mitotic spindle apparatus.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Research Program Projects (P01)
Project #
Application #
Study Section
Genetics Study Section (GEN)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Harvard University
Schools of Arts and Sciences
United States
Zip Code
Richter, B; Long, M; Lewontin, R C et al. (1997) Nucleotide variation and conservation at the dpp locus, a gene controlling early development in Drosophila. Genetics 145:311-23
Matthies, H J; McDonald, H B; Goldstein, L S et al. (1996) Anastral meiotic spindle morphogenesis: role of the non-claret disjunctional kinesin-like protein. J Cell Biol 134:455-64
Schaeffer, S W; Miller, E L (1992) Molecular population genetics of an electrophoretically monomorphic protein in the alcohol dehydrogenase region of Drosophila pseudoobscura. Genetics 132:163-78
Corbin, V; Michelson, A M; Abmayr, S M et al. (1991) A role for the Drosophila neurogenic genes in mesoderm differentiation. Cell 67:311-23
Schaeffer, S W; Miller, E L (1991) Nucleotide sequence analysis of Adh genes estimates the time of geographic isolation of the Bogota population of Drosophila pseudoobscura. Proc Natl Acad Sci U S A 88:6097-101
Michelson, A M; Abmayr, S M; Bate, M et al. (1990) Expression of a MyoD family member prefigures muscle pattern in Drosophila embryos. Genes Dev 4:2086-97
McDonald, H B; Stewart, R J; Goldstein, L S (1990) The kinesin-like ncd protein of Drosophila is a minus end-directed microtubule motor. Cell 63:1159-65
McDonald, H B; Goldstein, L S (1990) Identification and characterization of a gene encoding a kinesin-like protein in Drosophila. Cell 61:991-1000
Irminger-Finger, I; Laymon, R A; Goldstein, L S (1990) Analysis of the primary sequence and microtubule-binding region of the Drosophila 205K MAP. J Cell Biol 111:2563-72
Zhang, P; Knowles, B A; Goldstein, L S et al. (1990) A kinesin-like protein required for distributive chromosome segregation in Drosophila. Cell 62:1053-62

Showing the most recent 10 out of 24 publications