Abstract

1. Antisera raised in mice against purified nuclear and cytoplasmic poly(A)-binding proteins have been used to isolate genes for these proteins from yeast. DNA sequence determination and mutational analysis of the genes are in progess. 2. Preliminary studies have demonstrated the capacity of synthetic peptides to serve as signal sequences for the transport of proteins by nuclear membranes. We propose to exploit this finding to isolate the nuclear signal receptor, determine its location in the nuclear envelope, isolate the gene for the receptor, and reconstitute the transport process in vitro.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
5P01GM030387-08
Application #
3096126
Study Section
(SSS)
Project Start
1982-07-01
Project End
1991-06-30
Budget Start
1989-07-01
Budget End
1990-06-30
Support Year
8
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
Stanford University
Department
Type
Schools of Medicine
DUNS #
800771545
City
Stanford
State
CA
Country
United States
Zip Code
94305

  Publications

Park, B H; Levitt, M (1995) The complexity and accuracy of discrete state models of protein structure. J Mol Biol 249:493-507
Spudich, A; Braunstein, D (1995) Large secretory structures at the cell surface imaged with scanning force microscopy. Proc Natl Acad Sci U S A 92:6976-80
Hinds, D A; Levitt, M (1994) Exploring conformational space with a simple lattice model for protein structure. J Mol Biol 243:668-82
Lawlor, D A; Parham, P (1992) Structure of CD8 alpha and beta chains of the orangutan: novel patterns of mRNA splicing encoding hingeless polypeptides. Immunogenetics 36:121-5
Kubalek, E W; Kornberg, R D; Darst, S A (1991) Improved transfer of two-dimensional crystals from the air/water interface to specimen support grids for high-resolution analysis by electron microscopy. Ultramicroscopy 35:295-304
Darst, S A; Ahlers, M; Meller, P H et al. (1991) Two-dimensional crystals of streptavidin on biotinylated lipid layers and their interactions with biotinylated macromolecules. Biophys J 59:387-96
Tan, J L; Spudich, J A (1990) Dictyostelium myosin light chain kinase. Purification and characterization. J Biol Chem 265:13818-24
O'Halloran, T J; Spudich, J A (1990) Genetically engineered truncated myosin in Dictyostelium: the carboxyl-terminal regulatory domain is not required for the developmental cycle. Proc Natl Acad Sci U S A 87:8110-4
Meyer, T; Wensel, T; Stryer, L (1990) Kinetics of calcium channel opening by inositol 1,4,5-trisphosphate. Biochemistry 29:32-7
Meyer, T; Stryer, L (1990) Transient calcium release induced by successive increments of inositol 1,4,5-trisphosphate. Proc Natl Acad Sci U S A 87:3841-5

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