This is a renewal of a Program Project on the Molecular Basis of Biological Structure and Recognition. As before, the main goal of the Program Project is to bring together and strengthen the collaboration among four structural molecular biologists, in order to study problems in the structure, recognition and interactions of biological macromolecules and complexes. Physical techniques used include x-ray crystallography and two-dimensional nuclear magnetic resonance studies of nucleic acids and proteins, and computational biochemistry. Specific goals to be pursued include: (1) X-ray and NMR examination of synthetic DNA oligomers, and their complexes with antitumor drugs and proteins, the latter including the fis enhancer-binding protein of Salmonella and the TFIID TATA-binding protein of yeast [Dickerson, Feigon]. (2) Design of more efficient sequence-specific DNA-binding antitumor drugs, as vectors capable of differentiating between neoplastic and normal cells, or between invader and host cells [Dickerson] (3) Crystal structure analyses of the multi-subunit allosteric enzymes glutamine synthetase and ribulose bisphosphate carboxylase/oxygenase (RuBisCO), emphasizing the change in structures during the catalytic cycle and during regulation [Eisenberg]. (4) Protein design of aggregates of small peptides, followed by x-ray and NMR studies of their structures in the crystalline and solution states [Eisenberg, Feigon]. Redesign of the lac permease protein, to render it soluble and crystallizable [Eisenberg]. The long range goal of this effort is to learn and confirm rules of protein folding. (5) Correlation of protein sequences with their three-dimensional structures by means of 3D Structural Profiles. Computational studies of protein stability and antigenicity [Eisenberg, Yeates]. (6) Determination of the crystal structure of the oxygen-evolving enhancer protein 1 (OEEI), with an emphasis on understanding its interaction with the catalytic manganese cluster and with the photosynthetic reaction center. Prediction of residues important in photolysis and electron transfer [Yeates]. (7) Development of two new procedures for macromolecular crystal structure determination [Yeates]. (8) Maintenance and upgrading of the communal x-ray data collecting facility. (9) Maintenance and upgrading of the communal computer graphics facility used by all investigators. (10) Upgrading of the macromolecular NMR facility, built around a 500 MH NMR spectrometer.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
5P01GM031299-13
Application #
2176075
Study Section
Special Emphasis Panel (SSS (E))
Project Start
1983-04-01
Project End
1996-03-31
Budget Start
1995-04-01
Budget End
1996-03-31
Support Year
13
Fiscal Year
1995
Total Cost
Indirect Cost
Name
University of California Los Angeles
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095
Katsir, Galit; Jarvis, Michael; Phillips, Martin et al. (2015) The Escherichia coli NarL receiver domain regulates transcription through promoter specific functions. BMC Microbiol 15:174
Laganowsky, Arthur; Zhao, Minglei; Soriaga, Angela B et al. (2011) An approach to crystallizing proteins by metal-mediated synthetic symmetrization. Protein Sci 20:1876-90
Yeates, Todd O; Crowley, Christopher S; Tanaka, Shiho (2010) Bacterial microcompartment organelles: protein shell structure and evolution. Annu Rev Biophys 39:185-205
Boutz, Daniel R; Cascio, Duilio; Whitelegge, Julian et al. (2007) Discovery of a thermophilic protein complex stabilized by topologically interlinked chains. J Mol Biol 368:1332-44
Nelson, Rebecca; Eisenberg, David (2006) Recent atomic models of amyloid fibril structure. Curr Opin Struct Biol 16:260-5
Laidman, Janel; Forse, G Jason; Yeates, Todd O (2006) Conformational change and assembly through edge beta strands in transthyretin and other amyloid proteins. Acc Chem Res 39:576-83
Banatao, D Rey; Cascio, Duilio; Crowley, Christopher S et al. (2006) An approach to crystallizing proteins by synthetic symmetrization. Proc Natl Acad Sci U S A 103:16230-5
Norcross, Todd S; Yeates, Todd O (2006) A framework for describing topological frustration in models of protein folding. J Mol Biol 362:605-21
Maris, Ann E; Kaczor-Grzeskowiak, Maria; Ma, Zhongcai et al. (2005) Primary and secondary modes of DNA recognition by the NarL two-component response regulator. Biochemistry 44:14538-52
Nelson, Rebecca; Sawaya, Michael R; Balbirnie, Melinda et al. (2005) Structure of the cross-beta spine of amyloid-like fibrils. Nature 435:773-8

Showing the most recent 10 out of 127 publications