Abstract

The Core Laboratory goals for the next five-year grant period will be to; 1). Design to construct a new multi-channel NMR spectrometer operating at a 1H frequency of 750 MHZ. 2). Upgrade and maintain the two current 500 MHz spectrometers constructed with program project funds and owned by the program project. 3). Synthesize and purify routine DNA samples of varying g sequence for the participating laboratories in the program project. 4). Synthesize and purify DNA samples containing atom-specific and site- specific isotopic labels for members of the program project. The Core laboratory will continue to be a service facility to the entire six-lab Program Project.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
5P01GM032681-15
Application #
6107519
Study Section
Project Start
1998-01-01
Project End
1998-12-31
Budget Start
Budget End
Support Year
15
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of Washington
Department
Type
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Edfeldt, N B Fredrik; Harwood, Eric A; Sigurdsson, Snorri Th et al. (2004) Sequence context effect on the structure of nitrous acid induced DNA interstrand cross-links. Nucleic Acids Res 32:2795-801
Edfeldt, N B Fredrik; Harwood, Eric A; Sigurdsson, Snorri Th et al. (2004) Solution structure of a nitrous acid induced DNA interstrand cross-link. Nucleic Acids Res 32:2785-94
Okonogi, T M; Alley, S C; Reese, A W et al. (2002) Sequence-dependent dynamics of duplex DNA: the applicability of a dinucleotide model. Biophys J 83:3446-59
Okonogi, Tamara M; Alley, Stephen C; Harwood, Eric A et al. (2002) Phosphate backbone neutralization increases duplex DNA flexibility: a model for protein binding. Proc Natl Acad Sci U S A 99:4156-60
Hatcher, M E; LeTrong, I; Stenkamp, R et al. (2001) Local dynamics of the CpG step in a DNA crystal. J Am Chem Soc 123:8874-5
Meints, G A; Karlsson, T; Drobny, G P (2001) Modeling furanose ring dynamics in DNA. J Am Chem Soc 123:10030-8
Meints, G A; Drobny, G P (2001) Dynamic impact of methylation at the M. Hhai target site: a solid-state deuterium NMR study. Biochemistry 40:12436-43
Okonogi, T M; Alley, S C; Reese, A W et al. (2000) Sequence-dependent dynamics in duplex DNA. Biophys J 78:2560-71
Harwood, E A; Hopkins, P B; Sigurdsson, S T (2000) Chemical synthesis of cross-link lesions found in nitrous acid treated DNA: a general method for the preparation of N2-substituted 2'-deoxyguanosines. J Org Chem 65:2959-64
Geahigan, K B; Meints, G A; Hatcher, M E et al. (2000) The dynamic impact of CpG methylation in DNA. Biochemistry 39:4939-46

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