Molecular features of eukaryotic circadian rhythms are perhaps best described in the genetically tractable organism, Drosophila melanogaster. Yet there are only two identified clock components in Drosophila, the products of the two clock genes period and timeless. Based on the circadian cycle that these two proteins (PER and TIM) undergo, we propose to identify additional clock genes using molecular and biochemical approaches. These will comprise 1) cloning new rhythm genes identified in our ongoing behavioral screen, 2) identifying activities, proteins and then genes for rhythm-relevant kinases, phosphatases and protease, 3) identifying other potential rhythm and clock-regulated genes using differential expression procedures (subtraction and differential display), 4) identifying genes that encode relevant PER-and TIM- interacting proteins, and 5) identifying, purifying and cloning transcription factor activities that contribute to the robust transcriptional oscillations that the per and tim genes undergo.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
2P01GM033205-15
Application #
6271747
Study Section
Project Start
1998-07-01
Project End
1999-06-30
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
15
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Brandeis University
Department
Type
DUNS #
616845814
City
Waltham
State
MA
Country
United States
Zip Code
02454
Vodala, Sadanand; Pescatore, Stefan; Rodriguez, Joseph et al. (2012) The oscillating miRNA 959-964 cluster impacts Drosophila feeding time and other circadian outputs. Cell Metab 16:601-12
Shang, Yuhua; Haynes, Paula; Pírez, Nicolás et al. (2011) Imaging analysis of clock neurons reveals light buffers the wake-promoting effect of dopamine. Nat Neurosci 14:889-95
Hall, Jeffrey C (2005) Systems approaches to biological rhythms in Drosophila. Methods Enzymol 393:61-185
Choi, James C; Park, Demian; Griffith, Leslie C (2004) Electrophysiological and morphological characterization of identified motor neurons in the Drosophila third instar larva central nervous system. J Neurophysiol 91:2353-65
Busza, Ania; Emery-Le, Myai; Rosbash, Michael et al. (2004) Roles of the two Drosophila CRYPTOCHROME structural domains in circadian photoreception. Science 304:1503-6
Park, Demian; Coleman, Melissa J; Hodge, James J L et al. (2002) Regulation of neuronal excitability in Drosophila by constitutively active CaMKII. J Neurobiol 52:24-42
McDonald, M J; Rosbash, M; Emery, P (2001) Wild-type circadian rhythmicity is dependent on closely spaced E boxes in the Drosophila timeless promoter. Mol Cell Biol 21:1207-17
Joiner, M A; Griffith, L C (2000) Visual input regulates circuit configuration in courtship conditioning of Drosophila melanogaster. Learn Mem 7:32-42
Joiner, M A; Griffith, L C (1999) Mapping of the anatomical circuit of CaM kinase-dependent courtship conditioning in Drosophila. Learn Mem 6:177-92
DeSimone, S; Coelho, C; Roy, S et al. (1996) ERECT WING, the Drosophila member of a family of DNA binding proteins is required in imaginal myoblasts for flight muscle development. Development 122:31-9

Showing the most recent 10 out of 79 publications