Abstract

The principal role of the Core is to provide specialized protein reagents as needed for projects I-VI. Specifically the core funding will be used to prepare: (1). Native and recombinant human LBP and soluble CD14 (2). Anti-rabbit CD14 monoclonal and polyclonal antibody; anti-human CD14 monoclonal antibodies (3). Other recombinant proteins as needed for kinase assays or other in vitro biochemical assays (4). Other monoclonal or polyclonal antibodies as needed. These Core services will significantly free research personnel from the laborious task of preparing specialized protein reagents sot that more time can be devoted to experimental studies. In the past grant period we have found this mechanism to be a very time and cost-efficient method that we believe accounts in part for the rapid progress made in achieving the research aims of the previous grant period.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
5P01GM037696-14
Application #
6395884
Study Section
Project Start
2000-01-01
Project End
2000-12-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
14
Fiscal Year
2000
Total Cost
$158,879
Indirect Cost

  Institution

Name
Scripps Research Institute
Department
Type
DUNS #
City
La Jolla
State
CA
Country
United States
Zip Code
92037

  Publications

Tanino, Yoshinori; Chang, Mary Y; Wang, Xintao et al. (2012) Syndecan-4 regulates early neutrophil migration and pulmonary inflammation in response to lipopolysaccharide. Am J Respir Cell Mol Biol 47:196-202
Manukyan, Maria; Nalbant, Perihan; Luxen, Sylvia et al. (2009) RhoA GTPase activation by TLR2 and TLR3 ligands: connecting via Src to NF-kappa B. J Immunol 182:3522-9
Kurahashi, Kiyoyasu; Sawa, Teiji; Ota, Maria et al. (2009) Depletion of phagocytes in the reticuloendothelial system causes increased inflammation and mortality in rabbits with Pseudomonas aeruginosa pneumonia. Am J Physiol Lung Cell Mol Physiol 296:L198-209
Lu, Miao; Lin, Su-Chang; Huang, Yihua et al. (2007) XIAP induces NF-kappaB activation via the BIR1/TAB1 interaction and BIR1 dimerization. Mol Cell 26:689-702
Lombardo, Eleuterio; Alvarez-Barrientos, Alberto; Maroto, Beatriz et al. (2007) TLR4-mediated survival of macrophages is MyD88 dependent and requires TNF-alpha autocrine signalling. J Immunol 178:3731-9
da Silva Correia, J; Miranda, Y; Leonard, N et al. (2007) Regulation of Nod1-mediated signaling pathways. Cell Death Differ 14:830-9
Kang, Young Jun; Kim, Sung Ouk; Shimada, Shigeki et al. (2007) Cell surface 4-1BBL mediates sequential signaling pathways 'downstream'of TLR and is required for sustained TNF production in macrophages. Nat Immunol 8:601-9
Zhou, Huamin; Zheng, Min; Chen, Jianming et al. (2006) Determinants that control the specific interactions between TAB1 and p38alpha. Mol Cell Biol 26:3824-34
Lu, Gang; Kang, Young Jun; Han, Jiahuai et al. (2006) TAB-1 modulates intracellular localization of p38 MAP kinase and downstream signaling. J Biol Chem 281:6087-95
Kravchenko, Vladimir V; Kaufmann, Gunnar F; Mathison, John C et al. (2006) N-(3-oxo-acyl)homoserine lactones signal cell activation through a mechanism distinct from the canonical pathogen-associated molecular pattern recognition receptor pathways. J Biol Chem 281:28822-30

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