for use. The Biological Testing Core Facility is fully operational at present. We have acquired the necessary filtration apparatus, testing equipment for tadpoles and equipment for membrane preparations. The Research Unit shared facilities will be freely available for use, including centrifuges (low-, intermediate- and high-speed), cold room, gamma and scintillation counters and freezers. 2.F. LITERATURE CITED FOR THE BIOLOGICAL TESTING CORE FACILITY. [1] Covey, DF, Nathan, D, Kalkbrenner, M, Nilsson, KR, Hu, Y, Zorumski, CF and Evers, AS, Enantioselectivity of pregnanolone-induced gamma-aminobutyric acid(A) receptor modulation and anesthesia. Journal of Pharmacology and Experimental Therapeutics. 293:1009-1016, 2000. [2] Franks, NP and Lieb, WR, Selective actions of volatile general anaesthetics at molecular andcellular levels. British Journal of Anaesthesia. 71:65-76, 1993. [3] Gee, KW and Lan, NC, Gamma-aminobutyric acidA receptor complexes in rat frontal cortex and spinal cord show differential responses to steroid modulation. Molecular Pharmacology. 40:995- 999, 1991. [4] Halsey, MJ, Wardley-Smith, B and Wood, S, Pressure reversal of alphaxalone/alphadolone and methohexitone in tadpoles: evidence for different molecular sites for general anaesthesia. British Journal of Pharmacology. 89:299-305, 1986. [5] Majewska, MD, Harrison, NL, Schwartz, RD, Barker, JL and Paul, SM, Steroid hormone metabolites are barbiturate-like modulators of the GABA receptor. Science. 232:1004-1007, 1986. [6] Paradiso, K, Sabey, K, Evers, AS, Zorumski, CF, Covey, DF and Steinbach, JH, Steroid inhibition of rat neuronal nicotinic a4 B2 receptors expressed in HEK 293 cells. Molecular Pharmacology. 58:341-351,2000. [7] Srinivasan, S, Sapp, DW, Tobin, AJ and Olsen, RW, Biphasic modulation of GABA receptor binding by steroids suggests functional correlates. Neurochemical Research. 24:1363-1372, 1999. [8] Tonner, PH, Poppers, DM and Miller, KW, The general anesthetic potency of propofol and its dependence on hydrostatic pressure. Anesthesiology. 77:926-931, 1992. [9] Turner, DM, Ransom, RW, Yang, JS and Olsen, RW, Steroid anesthetics and naturally occurring analogs modulate the gamma-aminobutyric acid receptor complex at a

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
5P01GM047969-19
Application #
8118830
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
2010-08-01
Budget End
2011-07-31
Support Year
19
Fiscal Year
2010
Total Cost
$133,028
Indirect Cost
Name
Washington University
Department
Type
DUNS #
068552207
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
Budelier, Melissa M; Cheng, Wayland W L; Bergdoll, Lucie et al. (2017) Photoaffinity labeling with cholesterol analogues precisely maps a cholesterol-binding site in voltage-dependent anion channel-1. J Biol Chem 292:9294-9304
Jiang, Xiaoping; Shu, Hong-Jin; Krishnan, Kathiresan et al. (2016) A clickable neurosteroid photolabel reveals selective Golgi compartmentalization with preferential impact on proximal inhibition. Neuropharmacology 108:193-206
Li, Ping; Akk, Gustav (2015) Synaptic-type ?1?2?2L GABAA receptors produce large persistent currents in the presence of ambient GABA and anesthetic drugs. Mol Pharmacol 87:776-81
Zhou, Yu; Xia, Xiao-Ming; Lingle, Christopher J (2015) Cadmium-cysteine coordination in the BK inner pore region and its structural and functional implications. Proc Natl Acad Sci U S A 112:5237-42
Eaton, Megan M; Bracamontes, John; Shu, Hong-Jin et al. (2014) ?-aminobutyric acid type A ?4, ?2, and ? subunits assemble to produce more than one functionally distinct receptor type. Mol Pharmacol 86:647-56
Bracamontes, John R; Li, Ping; Akk, Gustav et al. (2014) Mutations in the main cytoplasmic loop of the GABA(A) receptor ?4 and ? subunits have opposite effects on surface expression. Mol Pharmacol 86:20-7
Zorumski, Charles F; Mennerick, Steven; Izumi, Yukitoshi (2014) Acute and chronic effects of ethanol on learning-related synaptic plasticity. Alcohol 48:1-17
Linsenbardt, Andrew J; Taylor, Amanda; Emnett, Christine M et al. (2014) Different oxysterols have opposing actions at N-methyl-D-aspartate receptors. Neuropharmacology 85:232-42
Jafurulla, Md; Rao, Bhagyashree D; Sreedevi, Sugunan et al. (2014) Stereospecific requirement of cholesterol in the function of the serotonin1A receptor. Biochim Biophys Acta 1838:158-63
Chen, Zi-Wei; Wang, Cunde; Krishnan, Kathiresan et al. (2014) 11-trifluoromethyl-phenyldiazirinyl neurosteroid analogues: potent general anesthetics and photolabeling reagents for GABAA receptors. Psychopharmacology (Berl) 231:3479-91

Showing the most recent 10 out of 193 publications