This Core will carry out five main and distinct activities: peptide synthesis, peptide analysis and characterization, mass spectrometric analysis of synthetic and native peptides, methodological developments as required by synthetic and analytical challenges and discovery projects. Peptide synthesis will be done using the solid phase approach and either Boc or Fmoc strategies. Peptide analysis and characterization will use HPLC, CZE, CD and MS. Methodological developments may include the synthesis of novel amino acids or the testing of novel supports for chromatography, among others. Mass spectrometry will be used independently to follow native conotoxins through their purification steps carried out at the University of Utah and the Salk Institute. Discovery projects will concentrate on the design of novel analogs of selected toxins using the aminoglycine and norcysteine scaffolds in addition to Ala-scans. This Core, by bringing together the synthetic and analytical expertise of an established group will enable members of this Program Project to reach their respective goals both economically and in a timely fashion. The Program Project Director and the PI of this Core set priorities and communicate regularly. Day to day operations will be coordinated by Drs. J. Rivier and W. Fischer at the Salk Institute and Dr. M. Mclntosh at the University of Utah.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
5P01GM048677-17
Application #
7929644
Study Section
Special Emphasis Panel (ZRG1)
Project Start
Project End
Budget Start
2009-07-01
Budget End
2010-06-30
Support Year
17
Fiscal Year
2009
Total Cost
$249,586
Indirect Cost
Name
University of Utah
Department
Type
DUNS #
009095365
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112
Yan, Yijin; Peng, Can; Arvin, Matthew C et al. (2018) Nicotinic Cholinergic Receptors in VTA Glutamate Neurons Modulate Excitatory Transmission. Cell Rep 23:2236-2244
Hone, Arik J; McIntosh, J Michael (2018) Nicotinic acetylcholine receptors in neuropathic and inflammatory pain. FEBS Lett 592:1045-1062
Hone, Arik J; Talley, Todd T; Bobango, Janet et al. (2018) Molecular determinants of ?-conotoxin potency for inhibition of human and rat ?6?4 nicotinic acetylcholine receptors. J Biol Chem 293:17838-17852
Banala, Sambashiva; Arvin, Matthew C; Bannon, Nicholas M et al. (2018) Photoactivatable drugs for nicotinic optopharmacology. Nat Methods 15:347-350
Hone, Arik J; Servent, Denis; McIntosh, J Michael (2018) ?9-containing nicotinic acetylcholine receptors and the modulation of pain. Br J Pharmacol 175:1915-1927
Espino, Samuel S; Robinson, Samuel D; Safavi-Hemami, Helena et al. (2018) Conopeptides promote itch through human itch receptor hMgprX1. Toxicon 154:28-34
Richter, Katrin; Sagawe, Sabrina; Hecker, Andreas et al. (2018) C-Reactive Protein Stimulates Nicotinic Acetylcholine Receptors to Control ATP-Mediated Monocytic Inflammasome Activation. Front Immunol 9:1604
Hiller, Sebastian Daniel; Heldmann, Sarah; Richter, Katrin et al. (2018) ?-Nicotinamide Adenine Dinucleotide (?-NAD) Inhibits ATP-Dependent IL-1? Release from Human Monocytic Cells. Int J Mol Sci 19:
Peng, Can; Yan, Yijin; Kim, Veronica J et al. (2018) Gene editing vectors for studying nicotinic acetylcholine receptors in cholinergic transmission. Eur J Neurosci :
Chen, De-Jie; Gao, Fen-Fei; Ma, Xiao-Kuang et al. (2018) Pharmacological and functional comparisons of ?6/?3?2?3-nAChRs and ?4?2-nAChRs heterologously expressed in the human epithelial SH-EP1 cell line. Acta Pharmacol Sin 39:1571-1581

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