This Program Project represents a coordinated and mutually supporting effort to pursue research in structural biology where electron diffraction and electron microscopy play a key role and to advance the technology that enables this research. Three projects within the Program represent applications of the methods of electron crystallography and microscopy, as well as of the instrumentation supported by the Program, to specific problems in structural biology. These applications include: Studies of the structure of tubulin and its interactions with ligands and other proteins that affect the dynamics of the microtubule cytoskeleton; Studies on condensins, proteins responsible for condensation of chromatin; Studies of the conformational changes that follow nucleotide hydrolysis in tubulin or that occur upon binding of drugs that inhibit polymerization. Additional, separately funded research projects which rely on equipment and facilities supported by the Program Project are also included as Associate member projects. The Administrative Core maintains the major equipment, supports computer resources, and provides a sense of intellectual community and common identity for all the participants in the Program Project.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
2P01GM051487-10A2
Application #
6912450
Study Section
Special Emphasis Panel (ZRG1-F05 (40))
Program Officer
Deatherage, James F
Project Start
1997-07-01
Project End
2010-04-30
Budget Start
2005-05-15
Budget End
2006-04-30
Support Year
10
Fiscal Year
2005
Total Cost
$1,693,921
Indirect Cost
Name
Lawrence Berkeley National Laboratory
Department
Neurosciences
Type
Organized Research Units
DUNS #
078576738
City
Berkeley
State
CA
Country
United States
Zip Code
94720
Zhang, Rui; LaFrance, Benjamin; Nogales, Eva (2018) Separating the effects of nucleotide and EB binding on microtubule structure. Proc Natl Acad Sci U S A 115:E6191-E6200
Nogales, Eva (2018) Cytoskeleton in high resolution. Nat Rev Mol Cell Biol 19:142
Downing, Kenneth H; Glaeser, Robert M (2018) Estimating the effect of finite depth of field in single-particle cryo-EM. Ultramicroscopy 184:94-99
Nogales, Eva (2018) Cryo-EM. Curr Biol 28:R1127-R1128
Sazzed, Salim; Song, Junha; Kovacs, Julio A et al. (2018) Tracing Actin Filament Bundles in Three-Dimensional Electron Tomography Density Maps of Hair Cell Stereocilia. Molecules 23:
Kamennaya, Nina A; Zemla, Marcin; Mahoney, Laura et al. (2018) High pCO2-induced exopolysaccharide-rich ballasted aggregates of planktonic cyanobacteria could explain Paleoproterozoic carbon burial. Nat Commun 9:2116
Howes, Stuart C; Geyer, Elisabeth A; LaFrance, Benjamin et al. (2018) Structural and functional differences between porcine brain and budding yeast microtubules. Cell Cycle 17:278-287
Glaeser, Robert M (2018) PROTEINS, INTERFACES, AND CRYO-EM GRIDS. Curr Opin Colloid Interface Sci 34:1-8
Kellogg, Elizabeth H; Hejab, Nisreen M A; Poepsel, Simon et al. (2018) Near-atomic model of microtubule-tau interactions. Science 360:1242-1246
Zhang, Rui; Roostalu, Johanna; Surrey, Thomas et al. (2017) Structural insight into TPX2-stimulated microtubule assembly. Elife 6:

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