The principal goal of this project is to develop uses of images derived from electron cryomicroscopy as molecular replacement models for x-ray crystallography. A second goal is to use high-resolution structures of viral proteins and subviral particles, together with cryoEM reconstructions of intact virions, to obtain detailed models for complex viruses. (1) Human papillomavirus 16 (HPV16) small virus-like particles (VLPs) will be used as test objects, to work out the best ways of determining the orientation and position parameters for placing a 3-D image reconstruction in the unit cell of a crystal and to analyze the resulting phase correlations in different resolution shells. We will analyze the effects of CTF corrections, procedures for deriving the reconstructed image, and molecular- replacement algorithms on the phase-determining power of the model. (2) Molecular replacement approaches will be used to solve the phase problem for rotavirus inner capsid particles (ICPs), for the rotavirus outer-shell protein, VP7, and for the retrovirus"""""""" penetration complex"""""""", mu13SIGMA3/3. 93) Crystal structures for components of retrovirus and rotavirus particles will be used, together with EM reconstructions of the intact viruses, to build models for the intact virions.
| Close, William; Neumann, Matthias; Schmidt, Andreas et al. (2018) Physical basis of amyloid fibril polymorphism. Nat Commun 9:699 |
| Loveland, Anna B; Demo, Gabriel; Grigorieff, Nikolaus et al. (2017) Ensemble cryo-EM elucidates the mechanism of translation fidelity. Nature 546:113-117 |
| Liu, Yuhang; Pan, Junhua; Jenni, Simon et al. (2017) CryoEM Structure of an Influenza Virus Receptor-Binding Site Antibody-Antigen Interface. J Mol Biol 429:1829-1839 |
| Abeyrathne, Priyanka D; Koh, Cha San; Grant, Timothy et al. (2016) Ensemble cryo-EM uncovers inchworm-like translocation of a viral IRES through the ribosome. Elife 5: |
| Schmidt, Andreas; Annamalai, Karthikeyan; Schmidt, Matthias et al. (2016) Cryo-EM reveals the steric zipper structure of a light chain-derived amyloid fibril. Proc Natl Acad Sci U S A 113:6200-5 |
| Chou, Hui-Ting; Dukovski, Danijela; Chambers, Melissa G et al. (2016) CATCHR, HOPS and CORVET tethering complexes share a similar architecture. Nat Struct Mol Biol 23:761-3 |
| Dimitrova, Yoana N; Jenni, Simon; Valverde, Roberto et al. (2016) Structure of the MIND Complex Defines a Regulatory Focus for Yeast Kinetochore Assembly. Cell 167:1014-1027.e12 |
| van der Feltz, Clarisse; Pomeranz Krummel, Daniel (2016) Purification of Native Complexes for Structural Study Using a Tandem Affinity Tag Method. J Vis Exp : |
| Loveland, Anna B; Bah, Eugene; Madireddy, Rohini et al. (2016) Ribosome•RelA structures reveal the mechanism of stringent response activation. Elife 5: |
| Baytshtok, Vladimir; Fei, Xue; Grant, Robert A et al. (2016) A Structurally Dynamic Region of the HslU Intermediate Domain Controls Protein Degradation and ATP Hydrolysis. Structure 24:1766-1777 |
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