The purpose of the Macromolecular Core is to provide a key common set of protein and RNA reagents to be used by the other components of the Program Project. The capabilities of the Macromolecular Core include peptide purification and labeling, RNA purification and labeling, isotopic labeling of RNA, and in vitro transcription of RNA. Reagents to provided by the core include: Full RRE RNA; Stem IIB RNA; Stem II RNA; 3'-biotinylation of RNA; Dye-labeling of synthetic RNAs; Isotopic and fluorine labeled RNAs; Rev protein, unlabeled and 15N-labeled; Rev protein mutants and deletions; and Dye-labeling of synthetic peptides. The Macromolecular Core consolidates the in-house expertise necessary to prepare these reagents, and facilitates interactions among the components by making a common set of reagents available to the program.
| Marenchino, Marco; Armbruster, David W; Hennig, Mirko (2009) Rapid and efficient purification of RNA-binding proteins: application to HIV-1 Rev. Protein Expr Purif 63:112-9 |
| Pond, Stephanie J K; Ridgeway, William K; Robertson, Rae et al. (2009) HIV-1 Rev protein assembles on viral RNA one molecule at a time. Proc Natl Acad Sci U S A 106:1404-8 |
| Edgcomb, Stephen P; Aschrafi, Angelique; Kompfner, Elizabeth et al. (2008) Protein structure and oligomerization are important for the formation of export-competent HIV-1 Rev-RRE complexes. Protein Sci 17:420-30 |
| Scott, Lincoln G; Hennig, Mirko (2008) RNA structure determination by NMR. Methods Mol Biol 452:29-61 |
| Carlomagno, Teresa; Amata, Irene; Williamson, James R et al. (2008) NMR assignments of HIV-2 TAR RNA. Biomol NMR Assign 2:167-9 |
| Pljevaljcic, Goran; Millar, David P (2008) Single-molecule fluorescence methods for the analysis of RNA folding and ribonucleoprotein assembly. Methods Enzymol 450:233-52 |
| Hennig, Mirko; Scott, Lincoln G; Sperling, Edit et al. (2007) Synthesis of 5-fluoropyrimidine nucleotides as sensitive NMR probes of RNA structure. J Am Chem Soc 129:14911-21 |
| Hennig, Mirko; Munzarova, Marketa L; Bermel, Wolfgang et al. (2006) Measurement of long-range 1H-19F scalar coupling constants and their glycosidic torsion dependence in 5-fluoropyrimidine-substituted RNA. J Am Chem Soc 128:5851-8 |
