Abstract

This Biochemical and Molecular Core facility, under the direction of Dr. David Harder with the assistance of Jayashree Narayanan, will provide a centralized professional service to all three projects involved in this Program. The staff will be trained by the project leaders or specialized personnel to perform a variety of procedures required in this Program Project Grant. This facility will maintain complete, computerized and centralized records and well-defined quality control guidelines for all assays. In addition new assays will be developed by Dr. Harder in consultation with investigators to meet the changing needs and directions of the Program. New assays that have thus far been developed by the Core include siRNA technology to silence gene expression, real time PCR to quantitate mRNA levels, metabolic models to record endothelial dysfunction and animal models to measure myocardial infarction. Dr. Harder and Ms. Narayanan have first hand experience in running core laboratories for two other Program Projects. The Core facility is located in the Cardiovascular Center which is in close proximity to the laboratories of the investigators for all three projects.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
5P01GM066730-08
Application #
8132320
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
2010-08-01
Budget End
2011-07-31
Support Year
8
Fiscal Year
2010
Total Cost
$292,770
Indirect Cost

  Institution

Name
Medical College of Wisconsin
Department
Type
DUNS #
937639060
City
Milwaukee
State
WI
Country
United States
Zip Code
53226

  Publications

Liu, Yong; Usa, Kristie; Wang, Feng et al. (2018) MicroRNA-214-3p in the Kidney Contributes to the Development of Hypertension. J Am Soc Nephrol 29:2518-2528
Zhang, Xiao; Dash, Ranjan K; Jacobs, Elizabeth R et al. (2018) Integrated computational model of the bioenergetics of isolated lung mitochondria. PLoS One 13:e0197921
Ghanian, Zahra; Konduri, Girija Ganesh; Audi, Said Halim et al. (2018) Quantitative optical measurement of mitochondrial superoxide dynamics in pulmonary artery endothelial cells. J Innov Opt Health Sci 11:
Liang, Mingyu (2018) Epigenetic Mechanisms and Hypertension. Hypertension 72:1244-1254
Pant, Tarun; Dhanasekaran, Anuradha; Fang, Juan et al. (2018) Current status and strategies of long noncoding RNA research for diabetic cardiomyopathy. BMC Cardiovasc Disord 18:197
Ge, Zhi-Dong; Li, Yingchuan; Qiao, Shigang et al. (2018) Failure of Isoflurane Cardiac Preconditioning in Obese Type 2 Diabetic Mice Involves Aberrant Regulation of MicroRNA-21, Endothelial Nitric-oxide Synthase, and Mitochondrial Complex I. Anesthesiology 128:117-129
Williams, Anna Marie; Liu, Yong; Regner, Kevin R et al. (2018) Artificial intelligence, physiological genomics, and precision medicine. Physiol Genomics 50:237-243
Liu, Pengyuan; Liu, Yong; Liu, Han et al. (2018) Role of DNA De Novo (De)Methylation in the Kidney in Salt-Induced Hypertension. Hypertension 72:1160-1171
Chuppa, Sandra; Liang, Mingyu; Liu, Pengyuan et al. (2018) MicroRNA-21 regulates peroxisome proliferator-activated receptor alpha, a molecular mechanism of cardiac pathology in Cardiorenal Syndrome Type 4. Kidney Int 93:375-389
Korman, Ben; Dash, Ranjan K; Peyton, Philip J (2018) Can Mathematical Modeling Explain the Measured Magnitude of the Second Gas Effect? Anesthesiology 128:1075-1083

Showing the most recent 10 out of 134 publications