Abstract

Projects 1 and 2 will require high-end optical microscopy with single molecule tracking and single vesicle fusion and FRET capabilities to achieve aims 1 and 2, and 1 and 3, respectively. Project 3 will measure interbilayer distances by FLIC microscopy (aim 1) and determine synaptotagmin-induced changes in lipid order by ATR-FTIR spectroscopy (aim 3). Core A will provide the necessary state-of-the-art instrumentation, technical know-how, and personnel training to realize these aims of all three Projects. In addition, the Core will train users, provide customable software tools, and assist with image and data analysis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
5P01GM072694-09
Application #
8706173
Study Section
Special Emphasis Panel (ZRG1)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
9
Fiscal Year
2014
Total Cost
Indirect Cost

  Institution

Name
University of Virginia
Department
Type
DUNS #
City
Charlottesville
State
VA
Country
United States
Zip Code
22904

  Publications

Yavuz, Halenur; Kattan, Iman; Hernandez, Javier M et al. (2018) Arrest of trans-SNARE zippering uncovers loosely and tightly docked intermediates in membrane fusion. J Biol Chem 293:8645-8655
Liang, Binyong; Tamm, Lukas K (2018) Solution NMR of SNAREs, complexin and ?-synuclein in association with membrane-mimetics. Prog Nucl Magn Reson Spectrosc 105:41-53
Hussain, Syed Saad; Harris, Megan T; Kreutzberger, Alex J B et al. (2018) Control of insulin granule formation and function by the ABC transporters ABCG1 and ABCA1 and by oxysterol binding protein OSBP. Mol Biol Cell 29:1238-1257
Blackburn, Matthew R; Hubbard, Caitlin; Kiessling, Volker et al. (2018) Distinct reaction mechanisms for hyaluronan biosynthesis in different kingdoms of life. Glycobiology 28:108-121
Witkowska, Agata; Jablonski, Lukasz; Jahn, Reinhard (2018) A convenient protocol for generating giant unilamellar vesicles containing SNARE proteins using electroformation. Sci Rep 8:9422
Kiessling, Volker; Kreutzberger, Alex J B; Liang, Binyong et al. (2018) A molecular mechanism for calcium-mediated synaptotagmin-triggered exocytosis. Nat Struct Mol Biol 25:911-917
Nyenhuis, Sarah B; Cafiso, David S (2018) Choice of reconstitution protocol modulates the aggregation state of full-length membrane-reconstituted synaptotagmin-1. Protein Sci 27:1008-1012
Katti, Sachin; Nyenhuis, Sarah B; Her, Bin et al. (2017) Non-Native Metal Ion Reveals the Role of Electrostatics in Synaptotagmin 1-Membrane Interactions. Biochemistry 56:3283-3295
Kiessling, Volker; Liang, Binyong; Kreutzberger, Alex J B et al. (2017) Planar Supported Membranes with Mobile SNARE Proteins and Quantitative Fluorescence Microscopy Assays to Study Synaptic Vesicle Fusion. Front Mol Neurosci 10:72
Kreutzberger, Alex J B; Kiessling, Volker; Liang, Binyong et al. (2017) Reconstitution of calcium-mediated exocytosis of dense-core vesicles. Sci Adv 3:e1603208

Showing the most recent 10 out of 76 publications