The purpose of this Mouse, Analytical and Cell Culture Core is to provide centralized care, breeding, genotyping and quality control of transgenic and knockout animals for use by investigators in the program project. In addition, the core will generate new transgenic and gene targeted mouse models where needed by program investigators. The core will also provide a mechanism for the distribution of new experimental transgenic and knockout models to either depository (such as the Jackson Laboratory Induced Mutant Resource) or to other investigators at the University of Iowa and outside the institution. Furthermore, the core will also provide centralized analyses of serum, urine and tissue extract for Na+, K+, CI-, Mg2+, Ca2+, phosphorous and creatinine. Additionally, it will generate immortalized cells and provide cells and tissues to each of the investigators of this Program Project. The overall Core is directed by Dr. Baoli Yang, who currently serves as Director of the Gene Targeting Core Facility on the campus at the University of Iowa.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
5P01GM078195-03
Application #
7843622
Study Section
Special Emphasis Panel (ZRG1)
Project Start
Project End
Budget Start
2009-05-01
Budget End
2010-04-30
Support Year
3
Fiscal Year
2009
Total Cost
$176,766
Indirect Cost
Name
University of Medicine & Dentistry of NJ
Department
Type
DUNS #
617022384
City
Piscataway
State
NJ
Country
United States
Zip Code
08854
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Huang, Junhao; Furuya, Hideki; Faouzi, Malika et al. (2017) Inhibition of TRPM7 suppresses cell proliferation of colon adenocarcinoma in vitro and induces hypomagnesemia in vivo without affecting azoxymethane-induced early colon cancer in mice. Cell Commun Signal 15:30
Antunes, Tayze T; Callera, Glaucia E; He, Ying et al. (2016) Transient Receptor Potential Melastatin 7 Cation Channel Kinase: New Player in Angiotensin II-Induced Hypertension. Hypertension 67:763-73
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Zierler, Susanna; Sumoza-Toledo, Adriana; Suzuki, Sayuri et al. (2016) TRPM7 kinase activity regulates murine mast cell degranulation. J Physiol 594:2957-70
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Zhang, Zheng; Yu, Haijie; Huang, Junhao et al. (2014) The TRPM6 kinase domain determines the Mg·ATP sensitivity of TRPM7/M6 heteromeric ion channels. J Biol Chem 289:5217-27
Chu, Hsueh-Ping; Liao, Yi; Novak, James S et al. (2014) Germline quality control: eEF2K stands guard to eliminate defective oocytes. Dev Cell 28:561-572
Fleig, Andrea; Chubanov, Vladimir (2014) TRPM7. Handb Exp Pharmacol 222:521-46

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