This human Embryonic Stem (hES) Cell Training and Culture Core has two objectives. The first is toprovide centralized hES culture support to all projects; the second is to broadly support hES cell work inour geographic region (Texas/Gulf Coast/South-west) by sponsoring a hES cell training program, tofacilitate adoption of the technology among a larger number of laboratories. For the first objective, we willprovide quality-tested reagents, such as lot-tested media and mouse embryonic fibroblasts (MEF) to allprojects. We will also provide cultures of WA01 (H1)and WA09 (H9)cells to project investigators on aroutine basis, and large-scale cultures of these lines upon request. We will ensure the hES provided toour investigators are high quality through routine testing for sterility, pluripotency, and genetic stability.For the second objective, we will offer a hES Training workshop every other month. This workshop willbe an intensive week-long hands-on training in small groups with highly experienced hES workers.Training will include all basic hES-related technologies, such as MEF preparation and quality testing, hESculture and quality analysis, teratoma formation, and EB differentiation. In addition, an extendedworkshop will be offered annually that will include strategies for genetic modification. This workshop willbe taught in collaboration with the Genetic Modification Core (Core C). By centralizing these efforts ofhES training and culture, we expect to ensure high quality hES culture for our investigators, as well asdissemination of the technology throughout the Texas Medical Center and larger geographic region.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Research Program Projects (P01)
Project #
1P01GM081627-01
Application #
7356526
Study Section
Special Emphasis Panel (ZGM1-GDB-8 (SC))
Project Start
Project End
Budget Start
2007-09-01
Budget End
2008-08-31
Support Year
1
Fiscal Year
2007
Total Cost
$268,625
Indirect Cost
Name
Baylor College of Medicine
Department
Type
DUNS #
051113330
City
Houston
State
TX
Country
United States
Zip Code
77030
Fujita, Jun; Freire, Pablo; Coarfa, Cristian et al. (2017) Ronin Governs Early Heart Development by Controlling Core Gene Expression Programs. Cell Rep 21:1562-1573
Jain, Abhinav K; Xi, Yuanxin; McCarthy, Ryan et al. (2016) LncPRESS1 Is a p53-Regulated LncRNA that Safeguards Pluripotency by Disrupting SIRT6-Mediated De-acetylation of Histone H3K56. Mol Cell 64:967-981
Wang, Hongran; Wang, Xiaohong; Xu, Xueping et al. (2016) Germ Cell Nuclear Factor (GCNF) Represses Oct4 Expression and Globally Modulates Gene Expression in Human Embryonic Stem (hES) Cells. J Biol Chem 291:8644-52
Wang, Hongran; Xi, Yutao; Zheng, Yi et al. (2016) Generation of electrophysiologically functional cardiomyocytes from mouse induced pluripotent stem cells. Stem Cell Res 16:522-30
Skinner, Samuel O; Xu, Heng; Nagarkar-Jaiswal, Sonal et al. (2016) Single-cell analysis of transcription kinetics across the cell cycle. Elife 5:e12175
Tang, Mengfan; Li, Yujing; Zhang, Yi et al. (2015) Disease mutant analysis identifies a new function of DAXX in telomerase regulation and telomere maintenance. J Cell Sci 128:331-41
Lu, Weisi; Fang, Lekun; Ouyang, Bin et al. (2015) Actl6a protects embryonic stem cells from differentiating into primitive endoderm. Stem Cells 33:1782-93
Zheng, Ze-Yi; Tian, Lin; Bu, Wen et al. (2015) Wild-Type N-Ras, Overexpressed in Basal-like Breast Cancer, Promotes Tumor Formation by Inducing IL-8 Secretion via JAK2 Activation. Cell Rep 12:511-24
Sabour, Davood; Xu, Xueping; Chung, Arthur C K et al. (2014) Germ cell nuclear factor regulates gametogenesis in developing gonads. PLoS One 9:e103985
Li, Yujing; Fong, Ka-Wing; Tang, Mengfan et al. (2014) Fam118B, a newly identified component of Cajal bodies, is required for Cajal body formation, snRNP biogenesis and cell viability. J Cell Sci 127:2029-39

Showing the most recent 10 out of 65 publications