Core A: Human Tissue Acquisition and Cell Culture LaboratoryINTRODUCTION. The availability of human tissues is a necessity for the conduct of experiments within eachof the projects, and the investigators collectively have many years of experience with experimentation involvinghuman tissue samples. The Program Project has for more than 20 years maintained an excellent workingrelationship with the Department of Pathology at our teaching hospital and with the staffs of the obstetric andgynecological services in the Department of Obstetrics and Gynecology. The current arrangement was built onthat historical base and modified as needed to accommodate specific needs of the projects and to keepabreast of compliance issues related to use and procurement of human tissues. Working with components ofthe Administrative Core, this centralized process for collection, processing, and culturing of human tissues andcells has proven to be e'<peditious and cost-effective. In addition, it provides a consistency in acquisition andprocessing and a standardization of tissue preparation and cell culturing that is extremely beneficial to theinvestigative process.A repository of frozen tissue samples is also maintained within this Core laboratory. The storedsamples include myometrium, endometrium, cervix, placenta, amnion, decidua, and cord blood plasmacollected over the last several years; also stored are samples of selected fetal tissues (12-20 weeksgestational age) collected during a previous program under an approved protocol for obtaining tissues fromelective abortions. A database is maintained of the stored tissues, their locations, and relevant collection andmedical information.In addition to obtaining tissues for use by Program Project investigators, the Department of Obstetricsand Gynecology is now able to fund partial salary support for research personnel so that this tissue acquisitionprogram is available to other investigators in the Department of Obstetrics and Gynecology as well as otherdepartments on campus, provided they have IRB-approved protocols.
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