Abstract

The major objective of the Tissue Culture Core is to coordinate services for the preparation of primary cultures of specific cell types that are required by all five individual projects. These cells include cortical astroglials, cortical neurons, cerebellar astrocytes, cerebellar granule cells, hippocampal pyramidal or dentate granule cells from rat brains and astroglial cells from fetal human brain. This core will also provide services for identifying and assessing the purity of the various cell types. In addition, it provides equipment, materials, training, service and technical expertise for cellular analysis that are required by the investigators. This core will be utilized 30 percent by Project I; 30 percent by Project II; 20 percent by Project III; 15 percent by Project IV; and five percent by Project V.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Program Projects (P01)
Project #
5P01HD016596-19
Application #
6607087
Study Section
Project Start
2002-05-01
Project End
2003-04-30
Budget Start
Budget End
Support Year
19
Fiscal Year
2002
Total Cost
Indirect Cost

  Institution

Name
University of Maryland Baltimore
Department
Type
DUNS #
003255213
City
Baltimore
State
MD
Country
United States
Zip Code
21201

  Publications

Jaber, Sausan M; Bordt, Evan A; Bhatt, Niraj M et al. (2018) Sex differences in the mitochondrial bioenergetics of astrocytes but not microglia at a physiologically relevant brain oxygen tension. Neurochem Int 117:82-90
Ferreira, Gustavo C; McKenna, Mary C (2017) L-Carnitine and Acetyl-L-carnitine Roles and Neuroprotection in Developing Brain. Neurochem Res 42:1661-1675
Tang, Shiyu; Xu, Su; Lu, Xin et al. (2016) Neuroprotective Effects of Acetyl-L-Carnitine on Neonatal Hypoxia Ischemia-Induced Brain Injury in Rats. Dev Neurosci 38:384-396
Demarest, Tyler G; Schuh, Rosemary A; Waddell, Jaylyn et al. (2016) Sex-dependent mitochondrial respiratory impairment and oxidative stress in a rat model of neonatal hypoxic-ischemic encephalopathy. J Neurochem 137:714-29
Waddell, Jaylyn; Hanscom, Marie; Shalon Edwards, N et al. (2016) Sex differences in cell genesis, hippocampal volume and behavioral outcomes in a rat model of neonatal HI. Exp Neurol 275 Pt 2:285-95
Demarest, Tyler G; McCarthy, Margaret M (2015) Sex differences in mitochondrial (dys)function: Implications for neuroprotection. J Bioenerg Biomembr 47:173-88
McKenna, Mary C; Rae, Caroline D (2015) A new role for ?-ketoglutarate dehydrogenase complex: regulating metabolism through post-translational modification of other enzymes. J Neurochem 134:3-6
Xu, Su; Waddell, Jaylyn; Zhu, Wenjun et al. (2015) In vivo longitudinal proton magnetic resonance spectroscopy on neonatal hypoxic-ischemic rat brain injury: Neuroprotective effects of acetyl-L-carnitine. Magn Reson Med 74:1530-42
Pershing, Michelle L; Bortz, David M; Pocivavsek, Ana et al. (2015) Elevated levels of kynurenic acid during gestation produce neurochemical, morphological, and cognitive deficits in adulthood: implications for schizophrenia. Neuropharmacology 90:33-41
McKenna, Mary C; Scafidi, Susanna; Robertson, Courtney L (2015) Metabolic Alterations in Developing Brain After Injury: Knowns and Unknowns. Neurochem Res 40:2527-43

Showing the most recent 10 out of 176 publications