Abstract

This Core is a tissue culture, protein purification and MHC typing core. In this facility we perform all sample processing that includes the following: isolation of peripheral blood lymphocytes, cryopreservation, thawing and washing of frozen cells and preparation of B cell lines. The core also performs the following tests: HLA-A, B, C typing by serology on all new samples as well as HLA-DR, DQ serology on a few selected samples. In addition, the core does DNA typing for HLA Class II alleles using sequence specific oligonucleotide probe hybridization (SSOPH). Other typings such as complement protein analysis are also performed on new samples. The Core also performs additional tests for other markers when necessary to confirm recombinant events. The Core will be responsible for the purification of the superantigens and some of the monoclonal antibodies used in some of the projects.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Program Projects (P01)
Project #
5P01HD017461-15
Application #
6108388
Study Section
Project Start
1998-04-01
Project End
2000-03-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
15
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
Immune Disease Institute, Inc.
Department
Type
DUNS #
115524410
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Szilagyi, Agnes; Banlaki, Zsofia; Pozsonyi, Eva et al. (2010) Frequent occurrence of conserved extended haplotypes (CEHs) in two Caucasian populations. Mol Immunol 47:1899-904
Johnson, B A; Geha, M; Blackwell, T K (2000) Similar but distinct effects of the tristetraprolin/TIS11 immediate-early proteins on cell survival. Oncogene 19:1657-64
Libert, C; Wielockx, B; Grijalba, B et al. (1999) The role of complement activation in tumour necrosis factor-induced lethal hepatitis. Cytokine 11:617-25
Circolo, A; Garnier, G; Fukuda, W et al. (1999) Genetic disruption of the murine complement C3 promoter region generates deficient mice with extrahepatic expression of C3 mRNA. Immunopharmacology 42:135-49
Lokki, M L; Circolo, A; Ahokas, P et al. (1999) Deficiency of human complement protein C4 due to identical frameshift mutations in the C4A and C4B genes. J Immunol 162:3687-93
De, J; Lai, W S; Thorn, J M et al. (1999) Identification of four CCCH zinc finger proteins in Xenopus, including a novel vertebrate protein with four zinc fingers and severely restricted expression. Gene 228:133-45
Hausmann, S; Biddison, W E; Smith, K J et al. (1999) Peptide recognition by two HLA-A2/Tax11-19-specific T cell clones in relationship to their MHC/peptide/TCR crystal structures. J Immunol 162:5389-97
Wang, X; Circolo, A; Lokki, M L et al. (1998) Molecular heterogeneity in deficiency of complement protein C2 type I. Immunology 93:184-91
Gauthier, L; Smith, K J; Pyrdol, J et al. (1998) Expression and crystallization of the complex of HLA-DR2 (DRA, DRB1*1501) and an immunodominant peptide of human myelin basic protein. Proc Natl Acad Sci U S A 95:11828-33
Tsitsikov, E N; Gutierrez-Ramos, J C; Geha, R S (1997) Impaired CD19 expression and signaling, enhanced antibody response to type II T independent antigen and reduction of B-1 cells in CD81-deficient mice. Proc Natl Acad Sci U S A 94:10844-9

Showing the most recent 10 out of 61 publications