Abstract

The investigator, proposes to analyze two actin binding proteins, the tropomyosin isoforms and caldesmon in regulating nonmuscle cell motility. Specifically (1) the role of nonmuscle tropomyosin isoforms in cytokinesis will be ascertained; (2) the effects of molecular manipulation of caldesmon on microfilament stability and motile behavior in mutants will be studied; (3) the role of tropomyosin isoforms and caldesmon in intracellular granule movements will be analyzed, by analyzing interaction of tropomyosin isoforms and caldesmon on interactions of actin and myosin I and actin and myosin V. Also (4) the regulation of tropomyosin isoforms will be extended to the Drosophila model in relation to transport and localization of maternal messages during embryogenesis. A combination of molecular biological, cell biological, biochemical; and immunological approaches will be used to study the role of the aforementioned proteins.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Program Projects (P01)
Project #
2P01HD018577-14
Application #
6240958
Study Section
Project Start
1997-04-01
Project End
1998-03-31
Budget Start
1996-10-01
Budget End
1997-09-30
Support Year
14
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
University of Iowa
Department
Type
DUNS #
041294109
City
Iowa City
State
IA
Country
United States
Zip Code
52242

  Publications

Soll, David R; Wessels, Deborah; Kuhl, Spencer et al. (2009) How a cell crawls and the role of cortical myosin II. Eukaryot Cell 8:1381-96
Lusche, Daniel F; Wessels, Deborah; Soll, David R (2009) The effects of extracellular calcium on motility, pseudopod and uropod formation, chemotaxis, and the cortical localization of myosin II in Dictyostelium discoideum. Cell Motil Cytoskeleton 66:567-87
Wessels, Deborah; Kuhl, Spencer; Soll, David R (2009) 2D and 3D quantitative analysis of cell motility and cytoskeletal dynamics. Methods Mol Biol 586:315-35
Lin, Jim Jung-Ching; Li, Yan; Eppinga, Robbin D et al. (2009) Chapter 1: roles of caldesmon in cell motility and actin cytoskeleton remodeling. Int Rev Cell Mol Biol 274:1-68
Wessels, Deborah J; Kuhl, Spencer; Soll, David R (2009) Light microscopy to image and quantify cell movement. Methods Mol Biol 571:455-71
Ueda, Atsushi; Wu, Chun-Fang (2009) Effects of social isolation on neuromuscular excitability and aggressive behaviors in Drosophila: altered responses by Hk and gsts1, two mutations implicated in redox regulation. J Neurogenet 23:378-94
Ueda, Atsushi; Wu, Chun-Fang (2009) Role of rut adenylyl cyclase in the ensemble regulation of presynaptic terminal excitability: reduced synaptic strength and precision in a Drosophila memory mutant. J Neurogenet 23:185-99
Ueda, Atsushi; Wu, Chun-Fang (2008) Effects of hyperkinetic, a beta subunit of Shaker voltage-dependent K+ channels, on the oxidation state of presynaptic nerve terminals. J Neurogenet 22:1-13
Lee, J; Ueda, A; Wu, C-F (2008) Pre- and post-synaptic mechanisms of synaptic strength homeostasis revealed by slowpoke and shaker K+ channel mutations in Drosophila. Neuroscience 154:1283-96
Volk, A Paige Davis; Heise, Christine K; Hougen, Jami L et al. (2008) ClC-3 and IClswell are required for normal neutrophil chemotaxis and shape change. J Biol Chem 283:34315-26

Showing the most recent 10 out of 179 publications