Abstract

These proposed experiments will combine genetic, cell biological and physiological approaches to study the development and plasticity of neuronal connections. Cultured neurons from Drosophila mutants that express defects in axonal navigation, nerve terminal arborization and synaptic plasticity will be used to focus on studies of the dynamic regulation of the growth cone cytoskeleton, membrane vesicle cycling, and cell adhesion by second messengers and protein kinases. The DIAS analysis system will be used to detect abnormal motility and related behaviors of growth cones from mutant neurons, and a fourth aim will also use DIAS to identify abnormal locomotory behaviors of mutant fly larvae.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Program Projects (P01)
Project #
2P01HD018577-19
Application #
6592829
Study Section
Project Start
2002-05-16
Project End
2007-03-31
Budget Start
Budget End
Support Year
19
Fiscal Year
2002
Total Cost
$143,176
Indirect Cost

  Institution

Name
University of Iowa
Department
Type
DUNS #
041294109
City
Iowa City
State
IA
Country
United States
Zip Code
52242

  Publications

Ueda, Atsushi; Wu, Chun-Fang (2009) Role of rut adenylyl cyclase in the ensemble regulation of presynaptic terminal excitability: reduced synaptic strength and precision in a Drosophila memory mutant. J Neurogenet 23:185-99
Soll, David R; Wessels, Deborah; Kuhl, Spencer et al. (2009) How a cell crawls and the role of cortical myosin II. Eukaryot Cell 8:1381-96
Lusche, Daniel F; Wessels, Deborah; Soll, David R (2009) The effects of extracellular calcium on motility, pseudopod and uropod formation, chemotaxis, and the cortical localization of myosin II in Dictyostelium discoideum. Cell Motil Cytoskeleton 66:567-87
Wessels, Deborah; Kuhl, Spencer; Soll, David R (2009) 2D and 3D quantitative analysis of cell motility and cytoskeletal dynamics. Methods Mol Biol 586:315-35
Lin, Jim Jung-Ching; Li, Yan; Eppinga, Robbin D et al. (2009) Chapter 1: roles of caldesmon in cell motility and actin cytoskeleton remodeling. Int Rev Cell Mol Biol 274:1-68
Wessels, Deborah J; Kuhl, Spencer; Soll, David R (2009) Light microscopy to image and quantify cell movement. Methods Mol Biol 571:455-71
Ueda, Atsushi; Wu, Chun-Fang (2009) Effects of social isolation on neuromuscular excitability and aggressive behaviors in Drosophila: altered responses by Hk and gsts1, two mutations implicated in redox regulation. J Neurogenet 23:378-94
Ueda, Atsushi; Wu, Chun-Fang (2008) Effects of hyperkinetic, a beta subunit of Shaker voltage-dependent K+ channels, on the oxidation state of presynaptic nerve terminals. J Neurogenet 22:1-13
Lee, J; Ueda, A; Wu, C-F (2008) Pre- and post-synaptic mechanisms of synaptic strength homeostasis revealed by slowpoke and shaker K+ channel mutations in Drosophila. Neuroscience 154:1283-96
Volk, A Paige Davis; Heise, Christine K; Hougen, Jami L et al. (2008) ClC-3 and IClswell are required for normal neutrophil chemotaxis and shape change. J Biol Chem 283:34315-26

Showing the most recent 10 out of 179 publications