Radioimmunoassay - Core

Chatterton, Robert T.

Abstract

of the RIA core includes methods to be employed for assays of the several peptide and steroid hormones and cyclic AMP. The peptide hormones from rats and hamsters and GnRH receptor concentrations will be assayed in the laboratory in Evanston under Dr. Schwartz' direction. All steroid hormones, whether measured directly in serum or after chromatographic purification, cAMP, and peptide hormones from human subjects will be assayed in the laboratory in Chicago under the direction of Dr. Chatterton. Anti-rabbit gamma globulin serum will be produced in the laboratory on the Chicago campus and provided to both laboratories for peptide hormone assays. Antisera for RIA of progesterone, estradiol and testosterone will also be produced there. Uniform quality control preparations will be produced for all assays. All data generated in the RIA laboratories will be entered in the data base file in the IBM-XT computers located on each campus. Data will be filed according to Project and experiment. Quality control data for all assays will also be entered in a computer file for each type of assay weekly, and running means and CVs will be computed. The computers on each campus will communicate through the fiber optic lines installed for that purpose so that all data will be accessible for all investigators at all times. Gonadotropins and other peptides will be measured in a total of over 103,000 tubes and steroids and cAMP in an additional 25,000 tubes yearly.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
• Type: Research Program Projects (P01)
• Project #: 1P01HD021921-01A1
• Application #: 3942724
• Support Year: 1
• Fiscal Year: 1987

Institution

• Name: Northwestern University at Chicago
• City: Evanston
• State: IL
• Country: United States
• Zip Code: 60208

Publications

Que, Emily L; Duncan, Francesca E; Bayer, Amanda R et al. (2017) Zinc sparks induce physiochemical changes in the egg zona pellucida that prevent polyspermy. Integr Biol (Camb) 9:135-144

Vanorny, Dallas A; Mayo, Kelly E (2017) The role of Notch signaling in the mammalian ovary. Reproduction 153:R187-R204

Xiao, Shuo; Duncan, Francesca E; Bai, Lu et al. (2015) Size-specific follicle selection improves mouse oocyte reproductive outcomes. Reproduction 150:183-92

Que, Emily L; Bleher, Reiner; Duncan, Francesca E et al. (2015) Quantitative mapping of zinc fluxes in the mammalian egg reveals the origin of fertilization-induced zinc sparks. Nat Chem 7:130-9

Xiao, Shuo; Zhang, Jiyang; Romero, Megan M et al. (2015) In vitro follicle growth supports human oocyte meiotic maturation. Sci Rep 5:17323

Cordeiro, Marília H; Kim, So-Youn; Ebbert, Katherine et al. (2015) Geography of follicle formation in the embryonic mouse ovary impacts activation pattern during the first wave of folliculogenesis. Biol Reprod 93:88

Kong, Betty Y; Duncan, Francesca E; Que, Emily L et al. (2015) The inorganic anatomy of the mammalian preimplantation embryo and the requirement of zinc during the first mitotic divisions. Dev Dyn 244:935-47

Kim, So-Youn; Ebbert, Katherine; Cordeiro, Marilia H et al. (2015) Cell autonomous phosphoinositide 3-kinase activation in oocytes disrupts normal ovarian function through promoting survival and overgrowth of ovarian follicles. Endocrinology 156:1464-76

Hong, Young Pyo; Gleber, Sophie-Charlotte; O'Halloran, Thomas V et al. (2014) Alignment of low-dose X-ray fluorescence tomography images using differential phase contrast. J Synchrotron Radiat 21:229-34

Kong, B Y; Duncan, F E; Que, E L et al. (2014) Maternally-derived zinc transporters ZIP6 and ZIP10 drive the mammalian oocyte-to-egg transition. Mol Hum Reprod 20:1077-89