We propose a Program Project that addresses two crucial questions in vertebrate development: (1) how stable phenotypic differences arise in cell lineages of multicellular embryos, and (2) how migration and localization of different embryonic cell populations and neuronal processes is controlled. We propose to examine two cell populations--the neural crest and primary neurons-- in a live vertebrate embryo, the zebrafish, Brachydanio rerio, where development can be observed and characterized directly. The neural crest gives rise to a variety of cellular phenotypes, many with useful markers of terminal differentiation. Since crest cells, appear to disperse, localize and differentiate in response to developmental cues encountered very early in development, they are ideal for studying the role of embryonic environments in regulating cellular differentiation and morphogenetic behavior. Primary neurons underlie the earliest embryonic behaviors. They arise early in early in embryonic development, and are the first neurons to grown axons. The primary motor axons, which are the first axons to appear in the periphery, navigate through the same interstitial environment at the same time as crest cells. We will study these two systems using cellular, molecular and genetic approaches. First, to understand the relative roles of cell lineages and environment in specification of cell fate, we will determine the detailed lineages of individual crest cell precursors and we will analyze the migration and interactions of crest cells with their environment in living embryos. Second, we will use monoclonal antibodies to identify molecules that may determine neuronal specification. Using these antibodies as probes, we will isolate and ultimately characterize the genes that code for these developmentally important molecules. Finally, we propose to characterize genetic and developmental mechanisms that determine cell fates by generating and analyzing mutations that affect neural crest development.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Program Projects (P01)
Project #
5P01HD022486-03
Application #
3097049
Study Section
Maternal and Child Health Research Committee (HDMC)
Project Start
1987-02-01
Project End
1992-01-31
Budget Start
1989-02-01
Budget End
1990-01-31
Support Year
3
Fiscal Year
1989
Total Cost
Indirect Cost
Name
University of Oregon
Department
Type
Graduate Schools
DUNS #
948117312
City
Eugene
State
OR
Country
United States
Zip Code
97403
Ferreira, Carlos R; Xia, Zhi-Jie; Clément, Aurélie et al. (2018) A Recurrent De Novo Heterozygous COG4 Substitution Leads to Saul-Wilson Syndrome, Disrupted Vesicular Trafficking, and Altered Proteoglycan Glycosylation. Am J Hum Genet 103:553-567
Logan, Savannah L; Dudley, Christopher; Baker, Ryan P et al. (2018) Automated high-throughput light-sheet fluorescence microscopy of larval zebrafish. PLoS One 13:e0198705
Clément, Aurélie; Blanco-Sánchez, Bernardo; Peirce, Judy L et al. (2018) Cog4 is required for protrusion and extension of the epithelium in the developing semicircular canals. Mech Dev :
Parthasarathy, Raghuveer (2018) Monitoring microbial communities using light sheet fluorescence microscopy. Curr Opin Microbiol 43:31-37
Troll, Joshua V; Hamilton, M Kristina; Abel, Melissa L et al. (2018) Microbiota promote secretory cell determination in the intestinal epithelium by modulating host Notch signaling. Development 145:
Dona, Margo; Slijkerman, Ralph; Lerner, Kimberly et al. (2018) Usherin defects lead to early-onset retinal dysfunction in zebrafish. Exp Eye Res 173:148-159
Blanco-Sánchez, Bernardo; Clément, Aurélie; Fierro Jr, Javier et al. (2018) Grxcr1 Promotes Hair Bundle Development by Destabilizing the Physical Interaction between Harmonin and Sans Usher Syndrome Proteins. Cell Rep 25:1281-1291.e4
Rolig, Annah S; Sweeney, Emily Goers; Kaye, Lila E et al. (2018) A bacterial immunomodulatory protein with lipocalin-like domains facilitates host-bacteria mutualism in larval zebrafish. Elife 7:
Logan, Savannah L; Thomas, Jacob; Yan, Jinyuan et al. (2018) The Vibrio cholerae type VI secretion system can modulate host intestinal mechanics to displace gut bacterial symbionts. Proc Natl Acad Sci U S A 115:E3779-E3787
Ganz, J; Baker, R P; Hamilton, M K et al. (2018) Image velocimetry and spectral analysis enable quantitative characterization of larval zebrafish gut motility. Neurogastroenterol Motil 30:e13351

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