Abstract

The function of the chemistry core is three fold: first, to provide services of a routine nature such as preparation of buffers, performing assays, etc.; secondly, to assist the individual investigators in operating the equipment of the core facility; and thirdly, to provide expertise in various separation techniques. Since all of the research projects proposed in this application rely heavily on a variety of biochemical procedures, the chemistry core will economize on time, space, and money by preventing duplication of equipment, and by assuring the quality and standardization of assays. The chemistry core will be composed of two personnel, the core director (Dr. Neal Musto) who will oversee the facility and a research assistant who will be responsible for the day to day operation. In addition, a relatively small amount of money is requested for purchasing commonly used biochemicals, such as buffers and supplies for low pressure chromatography, SDS-PAGE, etc.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Program Projects (P01)
Project #
5P01HD024633-03
Application #
3757236
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
3
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
Georgetown University
Department
Type
DUNS #
049515844
City
Washington
State
DC
Country
United States
Zip Code
20057

  Publications

Chen, Y; Dicou, E; Djakiew, D (1997) Characterization of nerve growth factor precursor protein expression in rat round spermatids and the trophic effects of nerve growth factor in the maintenance of Sertoli cell viability. Mol Cell Endocrinol 127:129-36
Wu, S M; Blomberg, L A; Chan, W Y (1996) Recovery of unlabeled PCR product from polyacrylamide gel for sequencing. Biotechniques 21:358-60, 362
Ali, S; Ravindranath, N; Jia, M C et al. (1996) Expression of multiple c-kit receptor messenger ribonucleic acid transcripts during postnatal development of the rat testis. Biochem Biophys Res Commun 218:104-12
Dym, M; Jia, M C; Dirami, G et al. (1995) Expression of c-kit receptor and its autophosphorylation in immature rat type A spermatogonia. Biol Reprod 52:8-19
Wykes, S M; Nelson, J E; Visscher, D W et al. (1995) Coordinate expression of the PRM1, PRM2, and TNP2 multigene locus in human testis. DNA Cell Biol 14:155-61
Boujrad, N; Ogwuegbu, S O; Garnier, M et al. (1995) Identification of a stimulator of steroid hormone synthesis isolated from testis. Science 268:1609-12
Vornberger, W; Prins, G; Musto, N A et al. (1994) Androgen receptor distribution in rat testis: new implications for androgen regulation of spermatogenesis. Endocrinology 134:2307-16
Djakiew, D; Pflug, B; Dionne, C et al. (1994) Postnatal expression of nerve growth factor receptors in the rat testis. Biol Reprod 51:214-21
Ravindranath, N; Papadopoulos, V; Vornberger, W et al. (1994) Ultrastructural distribution of calcium in the rat testis. Biol Reprod 51:50-62
Onoda, M; Djakiew, D (1993) A 24,500 Da protein derived from rat germ cells is associated with sertoli cell secretory function. Biochem Biophys Res Commun 197:688-95

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