Whereas the milk of various species contains growth factors (GF's), such as epidermal growth factor (EGF) and insulin-like growth factors (IGF-I and -II), they are absent in infant feeding formulae. Biochemical and physiological studies in experimental animals indicates that milk is an important source of exogenous GF-s for the suckling. Since it is known that transforming growth factor alpha (TGFalpha) exhibits similar effects as EGF, comparative studies using TGFalpha will be included. We will study the potential to synthesize EGF, TGFalpha, IGF-I and -II of individual organs of suckling rats at various postnatal developmental stages and compare the organ level of their peptide GFs, as well as EGF- receptor levels and corresponding messenger RNA (mRNA). We will explore whether the levels of these peptides and corresponding mRNA depend on milk-borne GF's, using rats fed rat milk substitute (RMS) containing no GF or as supplemented with GF. Our previous experiments have demonstrated presence of various forms of EGF in milk, the stomach (so-called gastric form), and small intestine (so-called intestinal form) of suckling rats, therefore, we will compare their biological activity.
| Sørensen, Karen Kristine; Simon-Santamaria, Jaione; McCuskey, Robert S et al. (2015) Liver Sinusoidal Endothelial Cells. Compr Physiol 5:1751-74 |
| Kling, Pamela J; Willeitner, Andrea; Dvorak, Bohuslav et al. (2008) Enteral erythropoietin and iron stimulate erythropoiesis in suckling rats. J Pediatr Gastroenterol Nutr 46:202-7 |
| McCuskey, Robert S (2008) The hepatic microvascular system in health and its response to toxicants. Anat Rec (Hoboken) 291:661-71 |
| Kling, Pamela J; Taing, K Muy; Dvorak, Bohuslav et al. (2006) Insulin-like growth factor-I stimulates erythropoiesis when administered enterally. Growth Factors 24:218-23 |
| Dvorak, Bohuslav; Halpern, Melissa D; Holubec, Hana et al. (2004) Rat milk decreases necrotizing enterocolitis in a rat model. Adv Exp Med Biol 554:471-3 |
| Dvorak, Bohuslav; Fituch, Camellia C; Williams, Catherine S et al. (2004) Concentrations of epidermal growth factor and transforming growth factor-alpha in preterm milk. Adv Exp Med Biol 554:407-9 |
| Kling, Pamela J; Hutter, John J (2003) Hematologic abnormalities in severe neonatal necrotizing enterocolitis: 25 years later. J Perinatol 23:523-30 |
| Dvorak, Bohuslav; Fituch, Camellia C; Williams, Catherine S et al. (2003) Increased epidermal growth factor levels in human milk of mothers with extremely premature infants. Pediatr Res 54:15-9 |
| Halpern, Melissa D; Dominguez, Jessica A; Dvorakova, Katerina et al. (2003) Ileal cytokine dysregulation in experimental necrotizing enterocolitis is reduced by epidermal growth factor. J Pediatr Gastroenterol Nutr 36:126-33 |
| Halpern, Melissa D; Holubec, Hana; Dominguez, Jessica A et al. (2003) Hepatic inflammatory mediators contribute to intestinal damage in necrotizing enterocolitis. Am J Physiol Gastrointest Liver Physiol 284:G695-702 |
Showing the most recent 10 out of 42 publications
