The overall objective of the MR Spectrometry Core (C) is to develop and apply multinuclear Nuclear Magnetic Resonance (NMR) spectrometry and imaging core facilities for the study of hypoxic-ischemic brain damage in immature experimental animals. The spectroscopy component of the Core will develop NMR spectroscopy techniques to evaluate metabolic alterations which occur during and following cerebral hypoxia-ischemia in immature rats and newborn dogs. Spatially localized 31P and 1H NMR spectroscopy will be used to quantitate changes in lactate concentrations, energy metabolism, intracellular pH and free cytosolic magnesium concentrations. We have developed a new iterative method to determine more accurately intracellular pH and free magnesium (Mg++) concentrations using the 3 resonances of ATP. The imaging component of the Core will develop and apply magnetic resonance imaging (MRI) techniques to evaluate perinatal hypoxic-ischemic brain damage. Central to the research will be the characterization of the normal developing rat brain. Two imaging techniques will be used to elucidate the sites and severities of brain damage at different states of hypoxia-ischemia and recovery. These techniques include diffusion and T2-weighted spin echo imaging. Preliminary results indicate that diffusion imaging is a sensitive method for early detection of hypoxic- ischemic injury, while T2-weighted spin echo imaging more accurately monitors long-term damage. Animal movement restraining devices essential to obtaining reliable diffusion images will be continually designed, constructed and refined. These devices will be incorporated into the general probe design for spectroscopy or imaging for each research project (Projects 1,3,4)

Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
1995
Total Cost
Indirect Cost
Name
Pennsylvania State University
Department
Type
DUNS #
129348186
City
Hershey
State
PA
Country
United States
Zip Code
17033
Leroy, Claire; Pierre, Karin; Simpson, Ian A et al. (2011) Temporal changes in mRNA expression of the brain nutrient transporters in the lithium-pilocarpine model of epilepsy in the immature and adult rat. Neurobiol Dis 43:588-97
Sen, Ellora; Basu, Anirban; Willing, Lisa B et al. (2011) Pre-conditioning induces the precocious differentiation of neonatal astrocytes to enhance their neuroprotective properties. ASN Neuro 3:e00062
Simpson, Ian A; Carruthers, Anthony; Vannucci, Susan J (2007) Supply and demand in cerebral energy metabolism: the role of nutrient transporters. J Cereb Blood Flow Metab 27:1766-91
Jin, Yuxuan; Silverman, Ann-Judith; Vannucci, Susan J (2007) Mast cell stabilization limits hypoxic-ischemic brain damage in the immature rat. Dev Neurosci 29:373-84
Kremlev, Sergey G; Roberts, Rebecca L; Palmer, Charles (2007) Minocycline modulates chemokine receptors but not interleukin-10 mRNA expression in hypoxic-ischemic neonatal rat brain. J Neurosci Res 85:2450-9
Zhang, X; Surguladze, N; Slagle-Webb, B et al. (2006) Cellular iron status influences the functional relationship between microglia and oligodendrocytes. Glia 54:795-804
Nehlig, Astrid; Rudolf, Gabrielle; Leroy, Claire et al. (2006) Pentylenetetrazol-induced status epilepticus up-regulates the expression of glucose transporter mRNAs but not proteins in the immature rat brain. Brain Res 1082:32-42
Hurn, Patricia D; Vannucci, Susan J; Hagberg, Henrik (2005) Adult or perinatal brain injury: does sex matter? Stroke 36:193-5
Kremlev, Sergey G; Palmer, Charles (2005) Interleukin-10 inhibits endotoxin-induced pro-inflammatory cytokines in microglial cell cultures. J Neuroimmunol 162:71-80
Basu, Anirban; Lazovic, Jelena; Krady, J Kyle et al. (2005) Interleukin-1 and the interleukin-1 type 1 receptor are essential for the progressive neurodegeneration that ensues subsequent to a mild hypoxic/ischemic injury. J Cereb Blood Flow Metab 25:17-29

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