Core - Cell Biology and Physiology

Kashgarian, Michael

Abstract

Joint use of Core facilities is a crucial activity in the operation of a Program Project. This Cell Biology and Physiology Core brings in investigators of 4 projects to facilitate access to specialized technologies and newer methods.
The aims of this Core are to provide: 1) technical assistance and training in electrophysiologic and molecular biologic techniques and approaches to investigators and supporting staff; 2) central consultative service for interpretation of data; 3) centralized facility for confocal microscopy which includes the study of ionic fluxes (e.g. Ca++, H+) and cytoskeletal and membrane proteins in live and fixed tissues for measurements of protein synthesis, amino-acid incorporation etc. and 4) a facility for fabrication and utilization of patch clamp, ion-selective microelectrodes for intracellular and extracellular ionic measurements. We believe that this Core will foster interactions between investigators and results from one project may affect the subsequent design and protocols in others within the program as we detailed in the overall introduction of this program above.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
• Type: Research Program Projects (P01)
• Project #: 5P01HD032573-02
• Application #: 5212911
• Support Year: 2
• Fiscal Year: 1996

Publications

Azad, Priti; Zhao, Huiwen W; Cabrales, Pedro J et al. (2016) Senp1 drives hypoxia-induced polycythemia via GATA1 and Bcl-xL in subjects with Monge's disease. J Exp Med 213:2729-2744

Yao, Hang; Azad, Priti; Zhao, Huiwen W et al. (2016) The Na+/HCO3- co-transporter is protective during ischemia in astrocytes. Neuroscience 339:329-337

Jha, Aashish R; Zhou, Dan; Brown, Christopher D et al. (2016) Shared Genetic Signals of Hypoxia Adaptation in Drosophila and in High-Altitude Human Populations. Mol Biol Evol 33:501-17

Pamenter, Matthew E; Haddad, Gabriel G (2015) High-throughput cell death assays. Methods Mol Biol 1254:153-63

Gu, Xiang Q; Pamenter, Matthew E; Siemen, Detlef et al. (2014) Mitochondrial but not plasmalemmal BK channels are hypoxia-sensitive in human glioma. Glia 62:504-13

Gersten, Merril; Zhou, Dan; Azad, Priti et al. (2014) Wnt pathway activation increases hypoxia tolerance during development. PLoS One 9:e103292

Udpa, Nitin; Ronen, Roy; Zhou, Dan et al. (2014) Whole genome sequencing of Ethiopian highlanders reveals conserved hypoxia tolerance genes. Genome Biol 15:R36

Salameh, Ahlam Ibrahim; Ruffin, Vernon A; Boron, Walter F (2014) Effects of metabolic acidosis on intracellular pH responses in multiple cell types. Am J Physiol Regul Integr Comp Physiol 307:R1413-27

Yin, Songyue; Xue, Jin; Sun, Haidan et al. (2013) Quantitative evaluation of the mitochondrial proteomes of Drosophila melanogaster adapted to extreme oxygen conditions. PLoS One 8:e74011

Zhou, Dan; Udpa, Nitin; Ronen, Roy et al. (2013) Whole-genome sequencing uncovers the genetic basis of chronic mountain sickness in Andean highlanders. Am J Hum Genet 93:452-62