Each of the principal investigators for projects I-III is a recognized expert in human and non-human primate embryonic stem and germ cells. The Embryonic Stem Cell and Embryonic Germ Cell (""""""""Stem and Germ Cell"""""""") Core draws on this combined expertise, thereby providing a central quality assurance and quality control laboratory for all cellular resources generated by the three projects.
Three specific aims are proposed for this core in support of the three projects.
Aim 1 : Establish and maintain core resource of human and non-human primate embryonic stem cells for distribution to each of the projects and the other cores using good laboratory practice culture and frequent monitoring of pluripotency and aneuploidy.
Aim 2 : Establish and maintain core resource of non-human primate embryonic germ cells for distribution to each of the projects and the other cores using good laboratory practice culture and frequent monitoring of pluripotency and aneuploidy.
Aim 3 (Stem Cell Genetic and Epigenetic Resource) Maintain sets of human and nonhuman primate ES and EG cell lines that are well-characterized with respect to their genetic and epigenetic status (whether normal or abnormal) for use in each of the projects and the other cores. The fulfillment of each of the three aims will support the three projects and the other cores in providing for in vitro molecular and cellular analysis, generation of chimeras (in the case of non-human primate ES and EG cells);and analysis of developmental fates, both in vitro and in vivo (the latter in transplantation studies involving animal models). By combining the expertise and resources of the three project leaders this core maximizes the available resources and reduces duplication and overlap.

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Program Projects (P01)
Project #
5P01HD047675-05
Application #
7916439
Study Section
Special Emphasis Panel (ZHD1)
Project Start
Project End
Budget Start
2009-06-01
Budget End
2010-05-31
Support Year
5
Fiscal Year
2009
Total Cost
$641,749
Indirect Cost
Name
Magee-Women's Research Institute and Foundation
Department
Type
DUNS #
119132785
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213
Zhang, Xiong; Simerly, Calvin; Hartnett, Carrie et al. (2014) Src-family tyrosine kinase activities are essential for differentiation of human embryonic stem cells. Stem Cell Res 13:379-89
Cibelli, Jose; Emborg, Marina E; Prockop, Darwin J et al. (2013) Strategies for improving animal models for regenerative medicine. Cell Stem Cell 12:271-4
Easley 4th, Charles A; Simerly, Calvin R; Schatten, Gerald (2013) Stem cell therapeutic possibilities: future therapeutic options for male-factor and female-factor infertility? Reprod Biomed Online 27:75-80
Ben-Yehudah, Ahmi; Campanaro, Becki M; Wakefield, Laura M et al. (2013) Nicotine exposure during differentiation causes inhibition of N-myc expression. Respir Res 14:119
Easley 4th, Charles A; Miki, Toshio; Castro, Carlos A et al. (2012) Human amniotic epithelial cells are reprogrammed more efficiently by induced pluripotency than adult fibroblasts. Cell Reprogram 14:193-203
Stringari, Chiara; Edwards, Robert A; Pate, Kira T et al. (2012) Metabolic trajectory of cellular differentiation in small intestine by Phasor Fluorescence Lifetime Microscopy of NADH. Sci Rep 2:568
Tachibana, Masahito; Sparman, Michelle; Ramsey, Cathy et al. (2012) Generation of chimeric rhesus monkeys. Cell 148:285-95
Easley 4th, Charles A; Phillips, Bart T; McGuire, Megan M et al. (2012) Direct differentiation of human pluripotent stem cells into haploid spermatogenic cells. Cell Rep 2:440-6
Lee, Hyo-Sang; Ma, Hong; Juanes, Rita Cervera et al. (2012) Rapid mitochondrial DNA segregation in primate preimplantation embryos precedes somatic and germline bottleneck. Cell Rep 1:506-15
Stringari, Chiara; Sierra, Robert; Donovan, Peter J et al. (2012) Label-free separation of human embryonic stem cells and their differentiating progenies by phasor fluorescence lifetime microscopy. J Biomed Opt 17:046012

Showing the most recent 10 out of 54 publications