Abstract

A. Objective The overall objective of this core facility is to provide placental tissues and purified cytotrophoblast (CTB) cells from abnormal term/preterm and 1st trimester pregnancies to the three projects in this Program grant application. DMA from these placental tissues and CTB cells, as well as from normal term placentas collected in Kansas, will be genotyped in the Core for polymorphisms in HLA-G (Project I, II and Project III), in LILRB1 and LILRB2 (Project I), and in PDCD1 (the gene encoding PD-1) and B7H1 (Project II). The rationale for studying polymorphisms in these genes is provided in the individual projects.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
Type
Research Program Projects (P01)
Project #
5P01HD049480-04
Application #
8049075
Study Section
Special Emphasis Panel (ZHD1)
Project Start
Project End
Budget Start
2010-04-01
Budget End
2011-03-31
Support Year
4
Fiscal Year
2010
Total Cost
$216,874
Indirect Cost

  Institution

Name
University of Kansas
Department
Type
DUNS #
016060860
City
Kansas City
State
KS
Country
United States
Zip Code
66160

  Publications

Linscheid, C; Heitmann, E; Singh, P et al. (2015) Trophoblast expression of the minor histocompatibility antigen HA-1 is regulated by oxygen and is increased in placentas from preeclamptic women. Placenta 36:832-8
Levin, Albert M; Mathias, Rasika A; Huang, Lili et al. (2013) A meta-analysis of genome-wide association studies for serum total IgE in diverse study populations. J Allergy Clin Immunol 131:1176-84
Loisel, Dagan A; Billstrand, Christine; Murray, Kathleen et al. (2013) The maternal HLA-G 1597?C null mutation is associated with increased risk of pre-eclampsia and reduced HLA-G expression during pregnancy in African-American women. Mol Hum Reprod 19:144-52
Hunt, J S; Petroff, M G (2013) IFPA Senior Award Lecture: Reproductive immunology in perspective--reprogramming at the maternal-fetal interface. Placenta 34 Suppl:S52-5
Kshirsagar, S K; Alam, S M; Jasti, S et al. (2012) Immunomodulatory molecules are released from the first trimester and term placenta via exosomes. Placenta 33:982-90
Jassem, Raghed M; Shani, Wafaa Sadoon; Loisel, Dagan A et al. (2012) HLA-G polymorphisms and soluble HLA-G protein levels in women with recurrent pregnancy loss from Basrah province in Iraq. Hum Immunol 73:811-7
Ding, David; Scott, Nicole M; Thompson, Emma E et al. (2012) Increased protein-coding mutations in the mitochondrial genome of African American women with preeclampsia. Reprod Sci 19:1343-51
Holland, Olivia J; Linscheid, Caitlin; Hodes, Herbert C et al. (2012) Minor histocompatibility antigens are expressed in syncytiotrophoblast and trophoblast debris: implications for maternal alloreactivity to the fetus. Am J Pathol 180:256-66
Petroff, M G (2011) Review: Fetal antigens--identity, origins, and influences on the maternal immune system. Placenta 32 Suppl 2:S176-81
Taglauer, Elizabeth S; Adams Waldorf, Kristina M; Petroff, Margaret G (2010) The hidden maternal-fetal interface: events involving the lymphoid organs in maternal-fetal tolerance. Int J Dev Biol 54:421-30

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