The Core will provide facilities for the application of molecular biology, immunocytochemical and imaging techniques. To facilitate these goals the core will provide: 1) technical assistance for tissue preparation and cell culture for molecular biology techniques and immunohistochemistry, 2) provide technical assistance in specialized biochemical or immunohistochemical techniques, 3) ready access to specialized equipment, 4) cost effectiveness through centralized equipment and facilities, 5) sharing of knowledge between different investigators. The services, instruction and specialized equipment provided by the Core will play a critical role in the successful completion of the research projects in an efficient and effective manner.
The specific aims of the Molecular Biology, Immunocytochemistry and Imaging Core are: 1. To provide assistance and facilities to investigators for cell culture, gene transfer in cultured cells and in tissues, and help in the quantification of gene expression by Northern and Western blot analysis and to estimate the functionality of the transferred gene using appropriate biochemical assays. 2. To provide facilities for histochemical and fluorescence applications for the detection of reporter proteins and transgene products in cells and tissues. 3. To provide assistance in immunofluorescence determinations of cell type and nerve terminals specific transgene expression using multiple antibodies. 4. To actively collaborate with the PPG investigators in designing and executing experiments pertaining to measurements of intracellular cation homeostasis, membrane potential and redox state of the cell during stimulus-response coupling. This will generally involve procedures of microscopic video digital image analysis system, and confocal microscopy. 5. To provide facilities and assistance in molecular biology techniques for the estimation of mRNA levels by Northern analysis and RT-PCR. 6. The activities of the core will be directed towards fostered development of new technology as well as collaborative investigations of focused problems.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
2P01HL014388-31
Application #
6704855
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
2003-01-21
Project End
2007-12-31
Budget Start
2003-01-21
Budget End
2003-12-31
Support Year
31
Fiscal Year
2003
Total Cost
$48,345
Indirect Cost
Name
University of Iowa
Department
Type
DUNS #
062761671
City
Iowa City
State
IA
Country
United States
Zip Code
52242
Lane-Cordova, Abbi D; Kalil, Graziela Z; Wagner, Christopher J et al. (2018) Hemoglobin A1c and C-reactive protein are independently associated with blunted nocturnal blood pressure dipping in obesity-related prediabetes. Hypertens Res 41:33-38
Larson, Robert A; Chapleau, Mark W (2018) Increased cardiac sympathetic activity: Cause or compensation in vasovagal syncope? Clin Auton Res 28:265-266
Tong, Brian; Abosi, Oluchi; Schmitz, Samantha et al. (2018) Bipolar disorder and related mood states are not associated with endothelial function of small arteries in adults without heart disease. Gen Hosp Psychiatry 51:36-40
Zeng, Wei-Zheng; Marshall, Kara L; Min, Soohong et al. (2018) PIEZOs mediate neuronal sensing of blood pressure and the baroreceptor reflex. Science 362:464-467
DuBose, Lyndsey E; Boles Ponto, Laura L; Moser, David J et al. (2018) Higher Aortic Stiffness Is Associated With Lower Global Cerebrovascular Reserve Among Older Humans. Hypertension 72:476-482
Holwerda, Seth W; Luehrs, Rachel E; Gremaud, Allene L et al. (2018) Relative burst amplitude of muscle sympathetic nerve activity is an indicator of altered sympathetic outflow in chronic anxiety. J Neurophysiol 120:11-22
Sabharwal, Rasna; Mason, Bianca N; Kuburas, Adisa et al. (2018) Increased receptor activity-modifying protein 1 in the nervous system is sufficient to protect against autonomic dysregulation and hypertension. J Cereb Blood Flow Metab :271678X17751352
Harwani, Sailesh C (2018) Macrophages under pressure: the role of macrophage polarization in hypertension. Transl Res 191:45-63
Shaffer Jr, Joseph J; Johnson, Casey P; Fiedorowicz, Jess G et al. (2018) Impaired sensory processing measured by functional MRI in Bipolar disorder manic and depressed mood states. Brain Imaging Behav 12:837-847
Xue, Baojian; Beltz, Terry G; Guo, Fang et al. (2018) Sex differences in maternal gestational hypertension-induced sensitization of angiotensin II hypertension in rat offspring: the protective effect of estrogen. Am J Physiol Regul Integr Comp Physiol 314:R274-R281

Showing the most recent 10 out of 175 publications