The Core will provide facilities for the application of molecular biology, immunocytochemical and imaging techniques. To facilitate these goals the core will provide: 1) technical assistance for tissue preparation and cell culture for molecular biology techniques and immunohistochemistry, 2) provide technical assistance in specialized biochemical or immunohistochemical techniques, 3) ready access to specialized equipment, 4) cost effectiveness through centralized equipment and facilities, 5) sharing of knowledge between different investigators. The services, instruction and specialized equipment provided by the Core will play a critical role in the successful completion of the research projects in an efficient and effective manner.
The specific aims of the Molecular Biology, Immunocytochemistry and Imaging Core are: 1. To provide assistance and facilities to investigators for cell culture, gene transfer in cultured cells and in tissues, and help in the quantification of gene expression by Northern and Western blot analysis and to estimate the functionality of the transferred gene using appropriate biochemical assays. 2. To provide facilities for histochemical and fluorescence applications for the detection of reporter proteins and transgene products in cells and tissues. 3. To provide assistance in immunofluorescence determinations of cell type and nerve terminals specific transgene expression using multiple antibodies. 4. To actively collaborate with the PPG investigators in designing and executing experiments pertaining to measurements of intracellular cation homeostasis, membrane potential and redox state of the cell during stimulus-response coupling. This will generally involve procedures of microscopic video digital image analysis system, and confocal microscopy. 5. To provide facilities and assistance in molecular biology techniques for the estimation of mRNA levels by Northern analysis and RT-PCR. 6. The activities of the core will be directed towards fostered development of new technology as well as collaborative investigations of focused problems.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
Project #
Application #
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Iowa
Iowa City
United States
Zip Code
Holwerda, Seth W; Holland, Marshall T; Reddy, Chandan G et al. (2018) Femoral vascular conductance and peroneal muscle sympathetic nerve activity responses to acute epidural spinal cord stimulation in humans. Exp Physiol 103:905-915
Persons, Jane E; Coryell, William H; Solomon, David A et al. (2018) Mixed state and suicide: Is the effect of mixed state on suicidal behavior more than the sum of its parts? Bipolar Disord 20:35-41
Kopf, Phillip G; Phelps, Laura E; Schupbach, Chad D et al. (2018) Differential effects of long-term slow-pressor and subpressor angiotensin II on contractile and relaxant reactivity of resistance versus conductance arteries. Physiol Rep 6:
Zhang, Yu-Ping; Huo, Yan-Li; Fang, Zhi-Qin et al. (2018) Maternal high-fat diet acts on the brain to induce baroreflex dysfunction and sensitization of angiotensin II-induced hypertension in adult offspring. Am J Physiol Heart Circ Physiol 314:H1061-H1069
Johnson, Casey P; Christensen, Gary E; Fiedorowicz, Jess G et al. (2018) Alterations of the cerebellum and basal ganglia in bipolar disorder mood states detected by quantitative T1? mapping. Bipolar Disord 20:381-390
Hardy, Rachel N; Simsek, Zinar D; Curry, Brandon et al. (2018) Aging affects isoproterenol-induced water drinking, astrocyte density, and central neuronal activation in female Brown Norway rats. Physiol Behav 192:90-97
Lane-Cordova, Abbi D; Kalil, Graziela Z; Wagner, Christopher J et al. (2018) Hemoglobin A1c and C-reactive protein are independently associated with blunted nocturnal blood pressure dipping in obesity-related prediabetes. Hypertens Res 41:33-38
Larson, Robert A; Chapleau, Mark W (2018) Increased cardiac sympathetic activity: Cause or compensation in vasovagal syncope? Clin Auton Res 28:265-266
Tong, Brian; Abosi, Oluchi; Schmitz, Samantha et al. (2018) Bipolar disorder and related mood states are not associated with endothelial function of small arteries in adults without heart disease. Gen Hosp Psychiatry 51:36-40
Zeng, Wei-Zheng; Marshall, Kara L; Min, Soohong et al. (2018) PIEZOs mediate neuronal sensing of blood pressure and the baroreceptor reflex. Science 362:464-467

Showing the most recent 10 out of 175 publications