Abstract

CORE C. Protein Engineering Core The purposed of this core is provide projects with purified wild type and engineered proteins needed for proposed experiments. Primarily this involves the generation of engineered myosin fragments. In addition, proteins will be through a combination of heterologous expression using either baculovirus/SF9 cells or E. coli and through the purification of proteins from rabbit tissues.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL015835-27
Application #
6202164
Study Section
Project Start
1999-09-01
Project End
2000-08-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
27
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Wu, Shenping; Liu, Jun; Reedy, Mary C et al. (2012) Structural changes in isometrically contracting insect flight muscle trapped following a mechanical perturbation. PLoS One 7:e39422
Wu, Shenping; Liu, Jun; Reedy, Mary C et al. (2010) Electron tomography of cryofixed, isometrically contracting insect flight muscle reveals novel actin-myosin interactions. PLoS One 5:
Burkeen, A K; Maday, S L; Rybicka, K K et al. (2004) Disruption of Caenorhabditis elegans muscle structure and function caused by mutation of troponin I. Biophys J 86:991-1001
Liu, Jun; Reedy, Mary C; Goldman, Yale E et al. (2004) Electron tomography of fast frozen, stretched rigor fibers reveals elastic distortions in the myosin crossbridges. J Struct Biol 147:268-82
Takagi, Y; Shuman, H; Goldman, Y E (2004) Coupling between phosphate release and force generation in muscle actomyosin. Philos Trans R Soc Lond B Biol Sci 359:1913-20
Tregear, Richard T; Reedy, Mary C; Goldman, Yale E et al. (2004) Cross-bridge number, position, and angle in target zones of cryofixed isometrically active insect flight muscle. Biophys J 86:3009-19
Polyak, Erzsebet; Standiford, David M; Yakopson, Vladimir et al. (2003) Contribution of myosin rod protein to the structural organization of adult and embryonic muscles in Drosophila. J Mol Biol 331:1077-91
Shaw, M Alexander; Ostap, E Michael; Goldman, Yale E (2003) Mechanism of inhibition of skeletal muscle actomyosin by N-benzyl-p-toluenesulfonamide. Biochemistry 42:6128-35
Cheung, A; Dantzig, J A; Hollingworth, S et al. (2002) A small-molecule inhibitor of skeletal muscle myosin II. Nat Cell Biol 4:83-8
Houdusse, A; Sweeney, H L (2001) Myosin motors: missing structures and hidden springs. Curr Opin Struct Biol 11:182-94

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