Abstract

The Molecular Biology Division consists of a closely knit group of independent investigators, united by a common interest in the structure-function relations of membrane bound enzymes and in electron transport mechanisms. The present application is in support of the individual programs of three responsible investigators and of the core personnel and the facilities they share. The projected studies center around investigation of oxidation-reduction mechanisms, particularly in membrane-bound enzymes and in certain other flavoproteins. The research emphasis of the component projects is as follows: structure-function relations, biosynthesis and assembly of membrane bound respiratory enzymes; structure, kinetic mechanism and active site of flavoproteins, including flavocytochromes; molecular mechanism of fatty acid oxidation in heart; and structural elucidation of new covalently bound flavins and iron-sulfur clusters.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL016251-15
Application #
3097550
Study Section
Heart, Lung, and Blood Research Review Committee A (HLBA)
Project Start
1978-09-01
Project End
1989-08-31
Budget Start
1987-09-01
Budget End
1989-08-31
Support Year
15
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
University of California San Francisco
Department
Type
Schools of Medicine
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143

  Publications

Cecchini, Gary; Schroder, Imke; Gunsalus, Robert P et al. (2002) Succinate dehydrogenase and fumarate reductase from Escherichia coli. Biochim Biophys Acta 1553:140-57
Tornroth, Susanna; Yankovskaya, Victoria; Cecchini, Gary et al. (2002) Purification, crystallisation and preliminary crystallographic studies of succinate:ubiquinone oxidoreductase from Escherichia coli. Biochim Biophys Acta 1553:171-6
Ackrell, Brian A C (2002) Cytopathies involving mitochondrial complex II. Mol Aspects Med 23:369-84
Leger, C; Heffron, K; Pershad, H R et al. (2001) Enzyme electrokinetics: energetics of succinate oxidation by fumarate reductase and succinate dehydrogenase. Biochemistry 40:11234-45
Sablin, S O; Ramsay, R R (2001) Substrates but not inhibitors alter the redox potentials of monoamine oxidases. Antioxid Redox Signal 3:723-9
Maklashina, E; Rothery, R A; Weiner, J H et al. (2001) Retention of heme in axial ligand mutants of succinate-ubiquinone xxidoreductase (complex II) from Escherichia coli. J Biol Chem 276:18968-76
Chiu, H J; Johnson, E; Schroder, I et al. (2001) Crystal structures of a novel ferric reductase from the hyperthermophilic archaeon Archaeoglobus fulgidus and its complex with NADP+. Structure 9:311-9
Efimov, I; Cronin, C N; McIntire, W S (2001) Effects of noncovalent and covalent FAD binding on the redox and catalytic properties of p-cresol methylhydroxylase. Biochemistry 40:2155-66
Iverson, T M; Luna-Chavez, C; Schroder, I et al. (2000) Analyzing your complexes: structure of the quinol-fumarate reductase respiratory complex. Curr Opin Struct Biol 10:448-55
Luna-Chavez, C; Iverson, T M; Rees, D C et al. (2000) Overexpression, purification, and crystallization of the membrane-bound fumarate reductase from Escherichia coli. Protein Expr Purif 19:188-96

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