Core - Biologicals

Dickinson, Craig

Abstract

This Core provides essential support services and reagents in the most efficient and cost effective manner for the conduct of the Projects in meeting the overall goals of this Program Project. The services include: (i) the generation, selection, cloning and establishing stable hybridoma lines producing monoclonal antibodies for the projects, (ii) characterizing MAbs, maintaining hybridoma lines, production of MAbs, (iii) acquiring, establishing or of clean cloned cell lines require for projects, (iv) acquisition of human endothelial cells, (v) maintenance of vectors and vectors containing inserts as required, (vi) maintenance, growth and harvest of cell lines transformed to produce desired recombinant proteins and mutants proteins, (vii) purification of mutant proteins, (viii) isolation of reagent plasma proteins for projects, (ix) preparation of immunoabsorbents, (x) common needs as they arise.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Heart, Lung, and Blood Institute (NHLBI)
• Type: Research Program Projects (P01)
• Project #: 5P01HL016411-23
• Application #: 5213176
• Support Year: 23
• Fiscal Year: 1996

Publications

Kurakula, Kondababu; Koenis, Duco S; Herzik Jr, Mark A et al. (2018) Structural and cellular mechanisms of peptidyl-prolyl isomerase Pin1-mediated enhancement of Tissue Factor gene expression, protein half-life, and pro-coagulant activity. Haematologica 103:1073-1082

Mackman, Nigel; Davis, George E (2011) Blood coagulation and blood vessel development: is tissue factor the missing link? Arterioscler Thromb Vasc Biol 31:2364-6

Lin, Rui; Roychowdhury-Saha, Manami; Black, Chris et al. (2011) Control of RNA processing by a large non-coding RNA over-expressed in carcinomas. FEBS Lett 585:671-6

Niessen, Frank; Furlan-Freguia, Christian; Fernández, José A et al. (2009) Endogenous EPCR/aPC-PAR1 signaling prevents inflammation-induced vascular leakage and lethality. Blood 113:2859-66

Sen, Mehmet; Herzik, Mark; Craft, John W et al. (2009) Spectroscopic Characterization of Successive Phosphorylation of the Tissue Factor Cytoplasmic Region. Open Spectrosc J 3:58-64

Ruf, W; Furlan-Freguia, C; Niessen, F (2009) Vascular and dendritic cell coagulation signaling in sepsis progression. J Thromb Haemost 7 Suppl 1:118-21

van den Berg, Y W; van den Hengel, L G; Myers, H R et al. (2009) Alternatively spliced tissue factor induces angiogenesis through integrin ligation. Proc Natl Acad Sci U S A 106:19497-502

Zhang, Qing; Petersen, Helle H; Ostergaard, Henrik et al. (2009) Molecular dynamics simulations and functional characterization of the interactions of the PAR2 ectodomain with factor VIIa. Proteins 77:559-69

Zhang, Qing; Sanner, Michel; Olson, Arthur J (2009) Shape complementarity of protein-protein complexes at multiple resolutions. Proteins 75:453-67

Potty, Ajish S R; Kourentzi, Katerina; Fang, Han et al. (2009) Biophysical characterization of DNA aptamer interactions with vascular endothelial growth factor. Biopolymers 91:145-56