Abstract

The laboratory core provides centralized resources for recombinant protein production, protein purification and standard quality control of these regents for the individual projects. This is an existing core unit that has established all aspects of transfection, selection and large scale growth of mammalian cell lines for the recombinant production of Vit.K dependent coagulation factors VIIa and X. Expression of soluble TF and site specific mutants in E. coli or S. cerevisiae are established routine procedures, including refolding and purification to homogeneity for successful crystallization of these proteins [1,2]. Protocols for the purification of plasma proteins, as well as, for the antibody production and fragmentation are in place. This core thus centralizes the production of essential reagents used by the various projects.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL016411-28
Application #
6464656
Study Section
Project Start
2001-06-01
Project End
2002-05-31
Budget Start
Budget End
Support Year
28
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
Scripps Research Institute
Department
Type
DUNS #
City
La Jolla
State
CA
Country
United States
Zip Code
92037

  Publications

Kurakula, Kondababu; Koenis, Duco S; Herzik Jr, Mark A et al. (2018) Structural and cellular mechanisms of peptidyl-prolyl isomerase Pin1-mediated enhancement of Tissue Factor gene expression, protein half-life, and pro-coagulant activity. Haematologica 103:1073-1082
Lin, Rui; Roychowdhury-Saha, Manami; Black, Chris et al. (2011) Control of RNA processing by a large non-coding RNA over-expressed in carcinomas. FEBS Lett 585:671-6
Mackman, Nigel; Davis, George E (2011) Blood coagulation and blood vessel development: is tissue factor the missing link? Arterioscler Thromb Vasc Biol 31:2364-6
Niessen, Frank; Furlan-Freguia, Christian; Fernández, José A et al. (2009) Endogenous EPCR/aPC-PAR1 signaling prevents inflammation-induced vascular leakage and lethality. Blood 113:2859-66
Sen, Mehmet; Herzik, Mark; Craft, John W et al. (2009) Spectroscopic Characterization of Successive Phosphorylation of the Tissue Factor Cytoplasmic Region. Open Spectrosc J 3:58-64
Ruf, W; Furlan-Freguia, C; Niessen, F (2009) Vascular and dendritic cell coagulation signaling in sepsis progression. J Thromb Haemost 7 Suppl 1:118-21
van den Berg, Y W; van den Hengel, L G; Myers, H R et al. (2009) Alternatively spliced tissue factor induces angiogenesis through integrin ligation. Proc Natl Acad Sci U S A 106:19497-502
Zhang, Qing; Petersen, Helle H; Ostergaard, Henrik et al. (2009) Molecular dynamics simulations and functional characterization of the interactions of the PAR2 ectodomain with factor VIIa. Proteins 77:559-69
Zhang, Qing; Sanner, Michel; Olson, Arthur J (2009) Shape complementarity of protein-protein complexes at multiple resolutions. Proteins 75:453-67
Potty, Ajish S R; Kourentzi, Katerina; Fang, Han et al. (2009) Biophysical characterization of DNA aptamer interactions with vascular endothelial growth factor. Biopolymers 91:145-56

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