This core is a necessary and integral component of this Program Project. It provides highlyexperienced technical expertise for the projects needed to meet the goals of the Program. Itprovides for the production and purification of the many recombinant proteins, wild-type ormodified, for the projects. This demands production by bacterial hosts as well as eukaryotic hosts such as CHO-K1 and variant cells that have been engineered for proper post-translationalmodification of proteins such as proper glycosylation, gamma carboxylation, proteolyticprocessing, analysis for purity and functional integrity. This core provides for gene transfer to cells cloning and expansion of these clones of modified cells. It provides for antisera to new proteins or specific for modifications of proteins. Also, it is very expert in hybridoma generation for monoclonal antibody production, selection, and establishing long-term high affinity and highlyspecific monoclonal antibody reagents for the projects where such reagents do not otherwise exist. The expertise is shared with investigators who are newly involved in the various technologies. The core personnel assist the various investigators in conduct of more sophisticated electrophoretic techniques and processing for mass spectroscopy. They supervise the proper use of common shared equipment. The core personnel are among the most expert personnel in sterile technique, cell culture, monoclonal antibody production, safety management, and recombinant protein production in the Institute.
Showing the most recent 10 out of 329 publications
