Cultured cells (primarily but not exclusively endothelial cells) are required by all four projects of this program project. This core will primarily serve the requirement of the three Rochester projects, although there will be some ongoing technology transfer in both directions between the core and Dr. Hamner's project. We anticipate that by combining much of the routine technical support for the culture systems we will be able to effect a significant saving in cost and effort. However, the major rationale for this core facility resides in our experience from the present project period that, for endothelial cells, there are stringent and differing requirements for growth the different ell types (HUVECs, HMEC-1s, BAECs) in the various growth configurations-microchannels, microslides, parallel plate systems etc.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL018208-28
Application #
6608261
Study Section
Project Start
2002-07-01
Project End
2003-06-30
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
28
Fiscal Year
2002
Total Cost
$109,973
Indirect Cost
Name
University of Rochester
Department
Type
DUNS #
208469486
City
Rochester
State
NY
Country
United States
Zip Code
14627
Vats, Kanika; Marsh, Graham; Harding, Kristen et al. (2017) Nanoscale physicochemical properties of chain- and step-growth polymerized PEG hydrogels affect cell-material interactions. J Biomed Mater Res A 105:1112-1122
Henry, Steven J; Crocker, John C; Hammer, Daniel A (2016) Motile Human Neutrophils Sense Ligand Density Over Their Entire Contact Area. Ann Biomed Eng 44:886-94
Marsh, Graham; Waugh, Richard E (2016) A simple approach for bioactive surface calibration using evanescent waves. J Microsc 262:245-51
Rocheleau, Anne D; Wang, Weiwei; King, Michael R (2016) Effect of Pseudopod Extensions on Neutrophil Hemodynamic Transport Near a Wall. Cell Mol Bioeng 9:85-95
Svetina, Saša; Kokot, Gašper; Kebe, Tjaša Švelc et al. (2016) A novel strain energy relationship for red blood cell membrane skeleton based on spectrin stiffness and its application to micropipette deformation. Biomech Model Mechanobiol 15:745-58
Rocheleau, Anne D; Cao, Thong M; Takitani, Tait et al. (2016) Comparison of human and mouse E-selectin binding to Sialyl-Lewis(x). BMC Struct Biol 16:10
MacKay, Joanna L; Hammer, Daniel A (2016) Stiff substrates enhance monocytic cell capture through E-selectin but not P-selectin. Integr Biol (Camb) 8:62-72
Hind, Laurel E; Lurier, Emily B; Dembo, Micah et al. (2016) Effect of M1-M2 Polarization on the Motility and Traction Stresses of Primary Human Macrophages. Cell Mol Bioeng 9:455-465
Hwang, G; Marsh, G; Gao, L et al. (2015) Binding Force Dynamics of Streptococcus mutans-glucosyltransferase B to Candida albicans. J Dent Res 94:1310-7
Henry, Steven J; Chen, Christopher S; Crocker, John C et al. (2015) Protrusive and Contractile Forces of Spreading Human Neutrophils. Biophys J 109:699-709

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