This research program continues to focus on serum lipoproteins considered as biochemical entities of significance in the etiology of atherosclerosis, lipid disorders and other diseases. Understanding such relationships requires the elucidation of the chemical, physical, and functional characteristics of the lipoproteins. Our research therefore emphasizes detailed characterization, as well as quantification, of serum lipoproteins in terms of classes and subclasses, distributions, interrelationships and origins. Our investigations of the lipoproteins will address a variety of problems and employ a broad scope of experimental techniques and skills. Among the principal tools that we expect to develop and apply to fundamental biophysical and biomedical problems are: analytic ultracentrifugation, electrophoresis, electron microscopy, chromatography, enzymatic methods and computer technology. A new apolipoprotein core unit will be directed toward determination of specific apolipoproteins. Lipoprotein structure and function will be examined in native and partially degraded particles and in physical or enzymatically reassembled model structures. Transformation in lipoprotein distributions will be studied in vitro as well as in vivo; the latter as a function of diet, drugs and disease. Human cord blood will be analyzed for information and insights into the genesis of lipoproteins. A major methodologic effort will be concerned with detection and minimization of potential artifacts and degradation arising from lipoprotein isolation and analysis. The unique methodologic resources of this Program Project will be available for outside collaborations on specialized or unique biomedical problems. The interactive design of developing methodology with application to basic and clinical research problems remains a major feature of our Program Project.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL018574-14
Application #
3097645
Study Section
Heart, Lung, and Blood Research Review Committee B (HLBB)
Project Start
1976-04-01
Project End
1991-03-31
Budget Start
1989-04-01
Budget End
1990-03-31
Support Year
14
Fiscal Year
1989
Total Cost
Indirect Cost
Name
Lawrence Berkeley National Laboratory
Department
Type
Organized Research Units
DUNS #
078576738
City
Berkeley
State
CA
Country
United States
Zip Code
94720
Ma, Ke; Forte, Trudy; Otvos, James D et al. (2005) Differential additive effects of endothelial lipase and scavenger receptor-class B type I on high-density lipoprotein metabolism in knockout mouse models. Arterioscler Thromb Vasc Biol 25:149-54
Kwiterovich Jr, Peter O; Cockrill, Steven L; Virgil, Donna G et al. (2005) A large high-density lipoprotein enriched in apolipoprotein C-I: a novel biochemical marker in infants of lower birth weight and younger gestational age. JAMA 293:1891-9
Dubrac, Sandrine; Lear, Steven R; Ananthanarayanan, Meena et al. (2005) Role of CYP27A in cholesterol and bile acid metabolism. J Lipid Res 46:76-85
Williams, Paul T; Blanche, Patricia J; Rawlings, Robin et al. (2005) Concordant lipoprotein and weight responses to dietary fat change in identical twins with divergent exercise levels 1. Am J Clin Nutr 82:181-7
Krauss, Ronald M (2005) Dietary and genetic probes of atherogenic dyslipidemia. Arterioscler Thromb Vasc Biol 25:2265-72
Badzioch, Michael D; Igo Jr, Robert P; Gagnon, France et al. (2004) Low-density lipoprotein particle size loci in familial combined hyperlipidemia: evidence for multiple loci from a genome scan. Arterioscler Thromb Vasc Biol 24:1942-50
Berneis, Kaspar; Shames, David M; Blanche, Patricia J et al. (2004) Plasma clearance of human low-density lipoprotein in human apolipoprotein B transgenic mice is related to particle diameter. Metabolism 53:483-7
Rizzo, Manfredi; Taylor, John M; Barbagallo, Carlo M et al. (2004) Effects on lipoprotein subclasses of combined expression of human hepatic lipase and human apoB in transgenic rabbits. Arterioscler Thromb Vasc Biol 24:141-6
Georgieva, A M; van Greevenbroek, M M J; Krauss, R M et al. (2004) Subclasses of low-density lipoprotein and very low-density lipoprotein in familial combined hyperlipidemia: relationship to multiple lipoprotein phenotype. Arterioscler Thromb Vasc Biol 24:744-9
Mar, Rebecca; Pajukanta, Paivi; Allayee, Hooman et al. (2004) Association of the APOLIPOPROTEIN A1/C3/A4/A5 gene cluster with triglyceride levels and LDL particle size in familial combined hyperlipidemia. Circ Res 94:993-9

Showing the most recent 10 out of 233 publications