We have identified a novel transmembrane protein tyrosine phosphatase (VPTPalpha) whose expression is highly regulated in rat vascular smooth muscle cells and in endothelial cells. In smooth muscle cells, expression level is correlated with the formation of """"""""epithelioid"""""""" monolayers in which adjacent cells neither overlap nor migrate away from each other. We hypothesize that VPTPalpha plays a role in regulating interactions which are involved in the development, maintenance and remodeling of the vessel wall. We will identify and characterize the extracellular """"""""ligand"""""""" for VPTPalpha. To obtain insight into the biochemical pathways in which VPTPalpha might function, we will identify cytoplasmic components which interact with the intracellular domain of VPTPalpha (substrates and effectors). We will evaluate the biological function of VPTPalpha by determining its pattern of expression in vivo and by evaluating the effects of experimentally manipulating its level and/or activity in cell culture and in vivo.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL018645-23S1
Application #
2823766
Study Section
Project Start
Project End
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
23
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of Washington
Department
Type
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195
Wight, Thomas N (2018) A role for proteoglycans in vascular disease. Matrix Biol 71-72:396-420
Kocarnik, Beverly M; Boyko, Edward J; Matsumoto, Alvin M et al. (2016) Baseline estradiol concentration in community-dwelling Japanese American men is not associated with intra-abdominal fat accumulation over 10 years. Obes Res Clin Pract 10:624-632
Nishizawa, Tomohiro; Kanter, Jenny E; Kramer, Farah et al. (2014) Testing the role of myeloid cell glucose flux in inflammation and atherosclerosis. Cell Rep 7:356-365
Wight, Thomas N; Kinsella, Michael G; Evanko, Stephen P et al. (2014) Versican and the regulation of cell phenotype in disease. Biochim Biophys Acta 1840:2441-51
Kang, Inkyung; Yoon, Dong Won; Braun, Kathleen R et al. (2014) Expression of versican V3 by arterial smooth muscle cells alters tumor growth factor ? (TGF?)-, epidermal growth factor (EGF)-, and nuclear factor ?B (NF?B)-dependent signaling pathways, creating a microenvironment that resists monocyte adhesion. J Biol Chem 289:15393-404
Ruppert, S M; Hawn, T R; Arrigoni, A et al. (2014) Tissue integrity signals communicated by high-molecular weight hyaluronan and the resolution of inflammation. Immunol Res 58:186-92
Wight, Thomas N; Kang, Inkyung; Merrilees, Mervyn J (2014) Versican and the control of inflammation. Matrix Biol 35:152-61
Tsubota, Yoshiaki; Frey, Jeremy M; Tai, Phillip W L et al. (2013) Monocyte ADAM17 promotes diapedesis during transendothelial migration: identification of steps and substrates targeted by metalloproteinases. J Immunol 190:4236-44
Cieslewicz, Maryelise; Tang, Jingjing; Yu, Jonathan L et al. (2013) Targeted delivery of proapoptotic peptides to tumor-associated macrophages improves survival. Proc Natl Acad Sci U S A 110:15919-24
Lund, Susan Amanda; Wilson, Carole L; Raines, Elaine W et al. (2013) Osteopontin mediates macrophage chemotaxis via ?4 and ?9 integrins and survival via the ?4 integrin. J Cell Biochem 114:1194-202

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