Project II is designed to identify pathways and targets in forebrain through which visceral signals received in the nucleus tractus solitarius (NTS) act to influence electrocortical activity, mood, and behavior. Specifically, the hypothesis will be tested that visceral stimuli research the cerebral cortex, particularly prefrontal regions, by projections from NTS to the midline- intralaminar thalamic complex (MITC). Study I is designed to address whether visceral afferents reach the MITC, disynaptically, via the NTS. Evidence will be sought using axonal tracers to, first define the visceral thalamus and to determine whether visceral-thalamic circuit neurons (l) are organized viscerotopially in NTS, and (ii) are characterized by neurotransmitter specificity, and (iii) receive synaptic input from primary visceral afferents, as established by electron microscopy (EM). Study II uses a comparable strategy to determine whether the NTS projects to the MITC, indirectly, by way of neurons in the rostral ventrolateral medulla (n.RVL). Evidence will be sought to determine whether projections from n.RVL to thalamus are catecholaminergic and arise from subsets of C1 neurons. Study III investigates whether neurons in NTS and n.RVL relay by way of MITC to viscerolimbic areas of prefrontal and cingulate cortex, hippocampus, or striatum. Experiments will evaluate (l) if targets within MITC relate topographically to afferent sources, particularly the NTS and n.RVL; and (ii) by EM, whether (a) the NTS terminates on thalamic neurons innervating the medial prefrontal cortex and (b) adrenergic neurons synapse in the visceral thalamus. In Study IV, a structural basis will be sought for parallel processing of visceral afferents by investigating whether visceral-thalamic circuit neurons branch collaterals to other major areas of relevance in emotional and autonomic processing. Dual- retrograde transport methods are used to determine whether the visceral thalamus is innervated by axon collaterals of neurons terminating in cerebral cortex, the amygdala and nucleus accumbens, or other sites in thalamus. Another question to be addressed is whether thalamic afferents issues collaterals to nuclei subserving autonomic/endocrine reflex regulation, the n.RVL, thoracic intermediolateral cell column or the paraventricular hypothalamic nucleus.

National Institute of Health (NIH)
National Heart, Lung, and Blood Institute (NHLBI)
Research Program Projects (P01)
Project #
Application #
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Weill Medical College of Cornell University
New York
United States
Zip Code
Glass, Michael J; Chan, June; Pickel, Virginia M (2017) Ultrastructural characterization of tumor necrosis factor alpha receptor type 1 distribution in the hypothalamic paraventricular nucleus of the mouse. Neuroscience 352:262-272
Takahashi, Reisuke H; Capetillo-Zarate, Estibaliz; Lin, Michael T et al. (2013) Accumulation of intraneuronal ýý-amyloid 42 peptides is associated with early changes in microtubule-associated protein 2 in neurites and synapses. PLoS One 8:e51965
Misono, K; Lessard, A (2012) Apomorphine-evoked redistribution of neurokinin-3 receptors in dopaminergic dendrites and neuronal nuclei of the rat ventral tegmental area. Neuroscience 203:27-38
Van Kempen, Tracey A; Milner, Teresa A; Waters, Elizabeth M (2011) Accelerated ovarian failure: a novel, chemically induced animal model of menopause. Brain Res 1379:176-87
Williams, Tanya J; Akama, Keith T; Knudsen, Margarete G et al. (2011) Ovarian hormones influence corticotropin releasing factor receptor colocalization with delta opioid receptors in CA1 pyramidal cell dendrites. Exp Neurol 230:186-96
Williams, T J; Milner, T A (2011) Delta opioid receptors colocalize with corticotropin releasing factor in hippocampal interneurons. Neuroscience 179:9-22
Williams, Tanya J; Torres-Reveron, Annelyn; Chapleau, Jeanette D et al. (2011) Hormonal regulation of delta opioid receptor immunoreactivity in interneurons and pyramidal cells in the rat hippocampus. Neurobiol Learn Mem 95:206-20
Spencer-Segal, Joanna L; Waters, Elizabeth M; Bath, Kevin G et al. (2011) Distribution of phosphorylated TrkB receptor in the mouse hippocampal formation depends on sex and estrous cycle stage. J Neurosci 31:6780-90
Williams, Tanya J; Mitterling, Katherine L; Thompson, Louisa I et al. (2011) Age- and hormone-regulation of opioid peptides and synaptic proteins in the rat dorsal hippocampal formation. Brain Res 1379:71-85
Takahashi, Reisuke H; Capetillo-Zarate, Estibaliz; Lin, Michael T et al. (2010) Co-occurrence of Alzheimer's disease ß-amyloid and ? pathologies at synapses. Neurobiol Aging 31:1145-52

Showing the most recent 10 out of 294 publications