Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL019259-10
Application #
4695519
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
10
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Massachusetts General Hospital
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
Jeffrey, P D; Schildbach, J F; Chang, C Y et al. (1995) Structure and specificity of the anti-digoxin antibody 40-50. J Mol Biol 248:344-60
Schildbach, J F; Shaw, S Y; Bruccoleri, R E et al. (1994) Contribution of a single heavy chain residue to specificity of an anti-digoxin monoclonal antibody. Protein Sci 3:737-49
Schunkert, H; Tang, S S; Litwin, S E et al. (1993) Regulation of intrarenal and circulating renin-angiotensin systems in severe heart failure in the rat. Cardiovasc Res 27:731-5
Jeffrey, P D; Strong, R K; Sieker, L C et al. (1993) 26-10 Fab-digoxin complex: affinity and specificity due to surface complementarity. Proc Natl Acad Sci U S A 90:10310-4
Love, T W; Quertermous, T; Zavodny, P J et al. (1993) High-level expression of antibody-plasminogen activator fusion proteins in hybridoma cells. Thromb Res 69:221-9
Schildbach, J F; Near, R I; Bruccoleri, R E et al. (1993) Heavy chain position 50 is a determinant of affinity and specificity for the anti-digoxin antibody 26-10. J Biol Chem 268:21739-47
Near, R I; Mudgett-Hunter, M; Novotny, J et al. (1993) Characterization of an anti-digoxin antibody binding site by site-directed in vitro mutagenesis. Mol Immunol 30:369-77
Schillbach, J F; Near, R I; Bruccoleri, R E et al. (1993) Modulation of antibody affinity by a non-contact residue. Protein Sci 2:206-14
Schunkert, H; Ingelfinger, J R; Hirsch, A T et al. (1993) Feedback regulation of angiotensin converting enzyme activity and mRNA levels by angiotensin II. Circ Res 72:312-8
Near, R I (1992) Gene conversion of immunoglobulin variable regions in mutagenesis cassettes by replacement PCR mutagenesis. Biotechniques 12:88-97

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