Abstract

The cell culture Core laboratory will serve as tissue culture resource for this Program Project. It will isolate primary cells for culture and maintain various cell lines. Type II cells isolated from neonatal and adult rats will be the principal cell type prepared. In addition, Core D will develop and characterize methods for the isolation and culture of pneumocytes which retain or recover important features of alveolar type II cells. especially those related to surfactant metabolism. The Core is located in the main IFEM laboratory and contains all equipment necessary for cell maintenance and propagation including incubators, laminar flow hoods,, inverted microscopes, a sterile room, and an autoclave. The personnel will include the Director, Dr. Sandra Bates, who has extensive experience in cell-culture, Jain-Qin Tao, the tissue culture technician, and Daisy Serrano, who will aid in cell culture and prepare media and glassware.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
2P01HL019737-26
Application #
6564816
Study Section
Project Start
2001-12-10
Project End
2006-11-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
26
Fiscal Year
2002
Total Cost
$223,655
Indirect Cost

  Institution

Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Hawkins, Arie; Guttentag, Susan H; Deterding, Robin et al. (2015) A non-BRICHOS SFTPC mutant (SP-CI73T) linked to interstitial lung disease promotes a late block in macroautophagy disrupting cellular proteostasis and mitophagy. Am J Physiol Lung Cell Mol Physiol 308:L33-47
Chowdhury, Ibrul; Fisher, Aron B; Christofidou-Solomidou, Melpo et al. (2014) Keratinocyte growth factor and glucocorticoid induction of human peroxiredoxin 6 gene expression occur by independent mechanisms that are synergistic. Antioxid Redox Signal 20:391-402
Roszell, Blair R; Tao, Jian-Qin; Yu, Kevin J et al. (2013) Pulmonary abnormalities in animal models due to Niemann-Pick type C1 (NPC1) or C2 (NPC2) disease. PLoS One 8:e67084
Roszell, Blair R; Tao, Jian-Qin; Yu, Kevin J et al. (2012) Characterization of the Niemann-Pick C pathway in alveolar type II cells and lamellar bodies of the lung. Am J Physiol Lung Cell Mol Physiol 302:L919-32
Rahaman, Hamidur; Zhou, Suiping; Dodia, Chandra et al. (2012) Increased phospholipase A2 activity with phosphorylation of peroxiredoxin 6 requires a conformational change in the protein. Biochemistry 51:5521-30
Weibel, Ginny L; Joshi, Michelle R; Jerome, W Gray et al. (2012) Cytoskeleton disruption in J774 macrophages: consequences for lipid droplet formation and cholesterol flux. Biochim Biophys Acta 1821:464-72
Maguire, Jean Ann; Mulugeta, Surafel; Beers, Michael F (2012) Multiple ways to die: delineation of the unfolded protein response and apoptosis induced by Surfactant Protein C BRICHOS mutants. Int J Biochem Cell Biol 44:101-12
Maguire, Jean Ann; Mulugeta, Surafel; Beers, Michael F (2011) Endoplasmic reticulum stress induced by surfactant protein C BRICHOS mutants promotes proinflammatory signaling by epithelial cells. Am J Respir Cell Mol Biol 44:404-14
Beers, Michael F; Hawkins, Arie; Maguire, Jean Ann et al. (2011) A nonaggregating surfactant protein C mutant is misdirected to early endosomes and disrupts phospholipid recycling. Traffic 12:1196-210
Zhang, Linghui; Yu, Kevin; Robert, Kyle W et al. (2011) Rab38 targets to lamellar bodies and normalizes their sizes in lung alveolar type II epithelial cells. Am J Physiol Lung Cell Mol Physiol 301:L461-77

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